| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.107ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This ATG7 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
ATG7 |
| Gene ID: |
10533 |
| Uniprot ID: |
ATG7_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. |
| Post Translational Modifications | Acetylated by EP300. |
| Function | E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Facilitates LC3-I lipidation with phosphatidylethanolamine to form LC3-II which is found on autophagosomal membranes. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Also plays a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner. |
| Protein Name | Ubiquitin-Like Modifier-Activating Enzyme Atg7Atg12-Activating Enzyme E1 Atg7Autophagy-Related Protein 7Apg7-LikeHagp7Ubiquitin-Activating Enzyme E1-Like Protein |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-6798695Reactome: R-HSA-6802952Reactome: R-HSA-983168 |
| Cellular Localisation | CytoplasmPreautophagosomal StructureLocalizes Also To Discrete Punctae Along The Ciliary Axoneme And To The Base Of The Ciliary Axoneme |
| Alternative ELISA Names | Ubiquitin-Like Modifier-Activating Enzyme Atg7 ELISA kitAtg12-Activating Enzyme E1 Atg7 ELISA kitAutophagy-Related Protein 7 ELISA kitApg7-Like ELISA kitHagp7 ELISA kitUbiquitin-Activating Enzyme E1-Like Protein ELISA kitATG7 ELISA kitAPG7L ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
