| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This ATF6 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
ATF6 |
| Gene ID: |
22926 |
| Uniprot ID: |
ATF6A_HUMAN |
| Specificity: |
This kit recognizes Human ATF6 in samples. No significant cross-reactivity or interference between Human ATF6 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | During unfolded protein response, a fragment of approximately 50 kDa containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) proteases. N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR). Ubiquitinated by RNF186 at Lys-152, which is required for pattern recognition receptor-induced unfolded protein response-associated outcomes. |
| Function | Cyclic AMP-dependent transcription factor ATF-6 alpha: Precursor of the transcription factor form (Processed cyclic AMP-dependent transcription factor ATF-6 alpha), which is embedded in the endoplasmic reticulum membrane. Endoplasmic reticulum stress promotes processing of this form, releasing the transcription factor form that translocates into the nucleus, where it activates transcription of genes involved in the unfolded protein response (UPR). Processed cyclic AMP-dependent transcription factor ATF-6 alpha: Transcription factor that initiates the unfolded protein response (UPR) during endoplasmic reticulum stress by activating transcription of genes involved in the UPR. Binds DNA on the 5'-CCACGA-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCACGA-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina. |
| Protein Name | Cyclic Amp-Dependent Transcription Factor Atf-6 AlphaCamp-Dependent Transcription Factor Atf-6 AlphaActivating Transcription Factor 6 AlphaAtf6-Alpha Cleaved Into - Processed Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha |
| Database Links | Reactome: R-HSA-380994Reactome: R-HSA-381033Reactome: R-HSA-381183 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Ii Membrane ProteinGolgi Apparatus MembraneTranslocates From The Endoplasmic Reticulum To The GolgiWhere It Is ProcessedProcessed Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha: NucleusUnder Er Stress The Cleaved N-Terminal Cytoplasmic Domain Translocates Into The NucleusThbs4 Promotes Its Nuclear Shuttling |
| Alternative ELISA Names | Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha ELISA kitCamp-Dependent Transcription Factor Atf-6 Alpha ELISA kitActivating Transcription Factor 6 Alpha ELISA kitAtf6-Alpha Cleaved Into - Processed Cyclic Amp-Dependent Transcription Factor Atf-6 Alpha ELISA kitATF6 ELISA kit |
| Specificity | This kit recognizes Human ATF6 in samples. No significant cross-reactivity or interference between Human ATF6 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-102 | 97 |
| EDTA plasma (n=8) | 93-107 | 101 |
| Cell culture media (n=8) | 90-105 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.11 | 2.35 | 9.88 | 1.08 | 2.36 | 9.90 |
| Standard deviation | 0.07 | 0.10 | 0.48 | 0.06 | 0.10 | 0.48 |
| CV (%) | 6.31 | 4.26 | 4.86 | 5.56 | 4.24 | 4.85 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
