| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This ARL1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
AKR1B10 |
| Gene ID: |
57016 |
| Uniprot ID: |
AK1BA_HUMAN |
| Specificity: |
This kit recognizes Human ARL1 in samples. No significant cross-reactivity or interference between Human ARL1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls). Displays no reductase activity towards glucose. |
| Protein Name | Aldo-Keto Reductase Family 1 Member B10Arl-1Aldose Reductase-LikeAldose Reductase-Related ProteinArpHarpSmall Intestine ReductaseSi Reductase |
| Database Links | Reactome: R-HSA-975634 |
| Cellular Localisation | LysosomeSecretedSecreted Through A Lysosome-Mediated Non-Classical Pathway |
| Alternative ELISA Names | Aldo-Keto Reductase Family 1 Member B10 ELISA kitArl-1 ELISA kitAldose Reductase-Like ELISA kitAldose Reductase-Related Protein ELISA kitArp ELISA kitHarp ELISA kitSmall Intestine Reductase ELISA kitSi Reductase ELISA kitAKR1B10 ELISA kitAKR1B11 ELISA kit |
| Specificity | This kit recognizes Human ARL1 in samples. No significant cross-reactivity or interference between Human ARL1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-97 | 91 |
| EDTA plasma (n=8) | 90-103 | 95 |
| Cell culture media (n=8) | 85-97 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 235.28 | 603.52 | 1722.01 | 249.22 | 662.01 | 1781.93 |
| Standard deviation | 13.72 | 34.04 | 76.11 | 12.61 | 30.25 | 83.39 |
| CV (%) | 5.83 | 5.64 | 4.42 | 5.06 | 4.57 | 4.68 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
