• Human APOA1 (Apolipoprotein A-I) CLIA Kit (STJC0000110)

Human APOA1 (Apolipoprotein A-I) CLIA Kit (STJC0000110)

SKU:
STJC0000110

£238.50 - £477.50
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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.47ng/mL
Detection Limit: 0.78~50ng/mL
Short Description: This human APOA1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: APOA1
Gene ID: 335
Uniprot ID: APOA1_HUMAN
Specificity: This kit recognizes Human ApoA1 in samples. No significant cross-reactivity or interference between Human ApoA1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.
Post Translational Modifications Glycosylated. Palmitoylated. Phosphorylation sites are present in the extracellular medium.
Function Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Protein Name Apolipoprotein A-I
Apo-Ai
Apoa-I
Apolipoprotein A1 Cleaved Into - Proapolipoprotein A-I
Proapoa-I - Truncated Apolipoprotein A-I
Apolipoprotein A-I(1-242
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-1369062
Reactome: R-HSA-1989781
Reactome: R-HSA-2168880
Reactome: R-HSA-3000471
Reactome: R-HSA-3000480
Reactome: R-HSA-381426
Reactome: R-HSA-5682113
Reactome: R-HSA-8957275
Reactome: R-HSA-8963888
Reactome: R-HSA-8963896
Reactome: R-HSA-8963901
Reactome: R-HSA-8964011
Reactome: R-HSA-8964058
Reactome: R-HSA-9707616
Reactome: R-HSA-975634
Reactome: R-HSA-977225
Cellular Localisation Secreted
Alternative CLIA Names Apolipoprotein A-I CLIA kit
Apo-Ai CLIA kit
Apoa-I CLIA kit
Apolipoprotein A1 Cleaved Into - Proapolipoprotein A-I CLIA kit
Proapoa-I - Truncated Apolipoprotein A-I CLIA kit
Apolipoprotein A-I(1-242 CLIA kit
APOA1 CLIA kit
Specificity This kit recognizes Human ApoA1 in samples. No significant cross-reactivity or interference between Human ApoA1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-103 94
EDTA plasma(n=8) 84-96 90
Cell culture media(n=8) 88-102 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.29 5.31 18.86 2.21 5.18 17.11
Standard deviation 0.28 0.63 1.32 0.28 0.41 1.71
CV (%) 12.23 11.86 7.00 12.67 7.92 9.99
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance