| Applications: | ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.064ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description: | This APAF1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | APAF1 |
| Gene ID: | 317 |
| Uniprot ID: | APAF_HUMAN |
| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. |
| Post Translational Modifications | |
| Function | Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. |
| Protein Name | Apoptotic Protease-Activating Factor 1Apaf-1 |
| Database Links | Reactome: R-HSA-111458Reactome: R-HSA-111459Reactome: R-HSA-111463Reactome: R-HSA-111464Reactome: R-HSA-6798695Reactome: R-HSA-6803207Reactome: R-HSA-8953750Reactome: R-HSA-9627069 |
| Cellular Localisation | Cytoplasm |
| Alternative ELISA Names | Apoptotic Protease-Activating Factor 1 ELISA kitApaf-1 ELISA kitAPAF1 ELISA kitKIAA0413 ELISA kit |
| output |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
