| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
75pg/mL |
| Detection Limit: |
125~8000pg/mL |
| Short Description: |
This AP13 Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
2.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
APLN |
| Gene ID: |
8862 |
| Uniprot ID: |
APEL_HUMAN |
| Specificity: |
This kit recognizes Human AP13 in samples. No significant cross-reactivity or interference between Human AP13 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Several active peptides may be produced by proteolytic processing of the peptide precursor. |
| Function | Endogenous ligand for the apelin receptor (APLNR). Drives internalization of the apelin receptor. Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR. Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis. Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking.the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates. Plays a role in early coronary blood vessels formation. Mediates myocardial contractility in an ERK1/2-dependent manner. May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior. (Microbial infection) Endogenous ligand for the apelin receptor (APLNR), an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APLNR. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. |
| Protein Name | ApelinApj Endogenous Ligand Cleaved Into - Apelin-36 - Apelin-31 - Apelin-28 - Apelin-13 |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-418594 |
| Cellular Localisation | SecretedExtracellular SpaceAbundantly Secreted In The ColostrumLower Level In MilkDecreases Rapidly Within Several Days After Parturition In MilkBut Is Still Detectable Even In Commercial Milk |
| Alternative ELISA Names | Apelin ELISA kitApj Endogenous Ligand Cleaved Into - Apelin-36 - Apelin-31 - Apelin-28 - Apelin-13 ELISA kitAPLN ELISA kitAPEL ELISA kit |
| Specificity | This kit recognizes AP13 in samples. No significant cross-reactivity or interference between AP13 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-103 | 98 |
| EDTA plasma(n=8) | 93-110 | 101 |
| Cell culture media(n=8) | 95-108 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 426.70 | 1074.70 | 3419.80 | 465.10 | 1177.90 | 3157.70 |
| Standard deviation | 28.20 | 51.60 | 143.60 | 31.60 | 56.50 | 132.60 |
| CV (%) | 6.61 | 4.80 | 4.20 | 6.79 | 4.80 | 4.20 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
