| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This ANXA1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
ANXA1 |
| Gene ID: |
301 |
| Uniprot ID: |
ANXA1_HUMAN |
| Specificity: |
This kit recognizes Human ANXA1 in samples. No significant cross-reactivity or interference between Human ANXA1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylated by protein kinase C, EGFR and TRPM7. Phosphorylated in response to EGF treatment. Sumoylated. Proteolytically cleaved by cathepsin CTSG to release the active N-terminal peptide Ac2-26. |
| Function | Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Has no effect on unstimulated T cells. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton. Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions. Displays Ca(2+)-dependent binding to phospholipid membranes. Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton. Annexin Ac2-26: Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Promotes resolution of inflammation and wound healing. Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2. |
| Protein Name | Annexin A1Annexin IAnnexin-1Calpactin IiCalpactin-2Chromobindin-9Lipocortin IPhospholipase A2 Inhibitory ProteinP35 Cleaved Into - Annexin Ac2-26 |
| Database Links | Reactome: R-HSA-416476Reactome: R-HSA-418594Reactome: R-HSA-444473Reactome: R-HSA-445355Reactome: R-HSA-6785807 |
| Cellular Localisation | NucleusCytoplasmCell ProjectionCiliumCell MembraneMembranePeripheral Membrane ProteinEndosome MembraneBasolateral Cell MembraneApical Cell MembraneLateral Cell MembraneSecretedExtracellular SpaceExtracellular SideExtracellular ExosomeCytoplasmic VesicleSecretory Vesicle LumenPhagocytic CupEarly EndosomeCytoplasmic Vesicle MembraneAt Least In Part Via Exosomes And Other Secretory VesiclesDetected In Exosomes And Other Extracellular VesiclesAlternativelyThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10It Results In The Protein Translocation From The Cytoplasm Into Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And SecretionDetected In Gelatinase Granules In Resting NeutrophilsSecretion Is Increased In Response To Wounding And InflammationSecretion Is Increased Upon T-Cell ActivationNeutrophil Adhesion To Endothelial Cells Stimulates Secretion Via Gelatinase GranulesBut Foreign Particle Phagocytosis Has No EffectColocalizes With Actin Fibers At Phagocytic CupsDisplays Calcium-Dependent Binding To Phospholipid Membranes |
| Alternative ELISA Names | Annexin A1 ELISA kitAnnexin I ELISA kitAnnexin-1 ELISA kitCalpactin Ii ELISA kitCalpactin-2 ELISA kitChromobindin-9 ELISA kitLipocortin I ELISA kitPhospholipase A2 Inhibitory Protein ELISA kitP35 Cleaved Into - Annexin Ac2-26 ELISA kitANXA1 ELISA kitANX1 ELISA kitLPC1 ELISA kit |
| Specificity | This kit recognizes Human ANXA1 in samples. No significant cross-reactivity or interference between Human ANXA1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-104 | 96 |
| EDTA plasma (n=8) | 91-108 | 99 |
| Cell culture media (n=8) | 94-107 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.91 | 2.01 | 9.28 | 0.88 | 2.07 | 9.54 |
| Standard deviation | 0.06 | 0.09 | 0.47 | 0.05 | 0.10 | 0.51 |
| CV (%) | 6.59 | 4.48 | 5.06 | 5.68 | 4.83 | 5.35 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
