| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.37ng/mL |
| Detection Limit: |
0.781-50ng/mL |
| Short Description: |
This ANXA1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Anxa1 |
| Gene ID: |
16952 |
| Uniprot ID: |
ANXA1_MOUSE |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | Detected in lung. Detected at the apical membrane of airway epithelial cells. Detected in intestinal epithelial cells. Detected in skeletal muscle. Detected in prostate. Detected in thymus (at protein level). Detected in stomach, lung, spleen, ovary and uterus, and at lower levels in kidney, thymus and heart. |
| Post Translational Modifications | Phosphorylated by protein kinase C, EGFR and TRPM7. Phosphorylated in response to EGF treatment. Sumoylated. Proteolytically cleaved by cathepsin CTSG to release the active N-terminal peptide Ac2-26. |
| Function | Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response. Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells. Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells. Has no effect on unstimulated T-cells. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton. Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions. Displays Ca(2+)-dependent binding to phospholipid membranes. Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton. Annexin Ac2-26: Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Promotes resolution of inflammation and wound healing. Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2. |
| Protein Name | Annexin A1Annexin IAnnexin-1Calpactin IiCalpactin-2Chromobindin-9Lipocortin IPhospholipase A2 Inhibitory ProteinP35 Cleaved Into - Annexin Ac2-26 |
| Database Links | Reactome: R-MMU-416476Reactome: -MMU-418594Reactome: -MMU-444473 |
| Cellular Localisation | NucleusCytoplasmCell ProjectionCiliumBasolateral Cell MembraneLateral Cell MembraneCell MembranePeripheral Membrane ProteinApical Cell MembraneMembraneEarly EndosomeCytoplasmic Vesicle MembraneEndosome MembraneSecretedExtracellular SpaceExtracellular SideExtracellular ExosomeCytoplasmic VesicleSecretory Vesicle LumenPhagocytic CupColocalizes With Actin Fibers At Phagocytic CupsAt Least In Part Via Exosomes And Other Secretory VesiclesDetected In Exosomes And Other Extracellular VesiclesSecretion Is Increased In Response To Wounding And InflammationDetected In Gelatinase Granules In Resting NeutrophilsNeutrophil Adhesion To Endothelial Cells Stimulates Secretion Via Gelatinase GranulesBut Foreign Particle Phagocytosis Has No EffectDisplays Calcium-Dependent Binding To Phospholipid Membranes |
| Alternative ELISA Names | Annexin A1 ELISA kitAnnexin I ELISA kitAnnexin-1 ELISA kitCalpactin Ii ELISA kitCalpactin-2 ELISA kitChromobindin-9 ELISA kitLipocortin I ELISA kitPhospholipase A2 Inhibitory Protein ELISA kitP35 Cleaved Into - Annexin Ac2-26 ELISA kitAnxa1 ELISA kitAnx1 ELISA kitLpc-1 ELISA kitLpc1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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