| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This Alpha MSH Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
2.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
POMC |
| Gene ID: |
5443 |
| Uniprot ID: |
COLI_HUMAN |
| Specificity: |
This kit recognizes Human Alpha MSH in samples. No significant cross-reactivity or interference between Human Alpha MSH and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Specific enzymatic cleavages at paired basic residues yield the different active peptides. O-glycosylated.reducing sugar is probably N-acetylgalactosamine. |
| Function | Corticotropin: Stimulates the adrenal glands to release cortisol. Melanocyte-stimulating hormone alpha: Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes. Melanocyte-stimulating hormone beta: Increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin: Endogenous orexigenic opiate. Met-enkephalin: Endogenous opiate. |
| Protein Name | Pro-OpiomelanocortinPomcCorticotropin-Lipotropin Cleaved Into - Npp - Melanotropin GammaGamma-Msh - Potential Peptide - CorticotropinAdrenocorticotropic HormoneActh - Melanocyte-Stimulating Hormone AlphaAlpha-MshMelanotropin Alpha - Corticotropin-Like Intermediary PeptideClip - Lipotropin BetaBeta-Lph - Lipotropin GammaGamma-Lph - Melanocyte-Stimulating Hormone BetaBeta-MshMelanotropin Beta - Beta-Endorphin - Met-Enkephalin |
| Database Links | Reactome: R-HSA-111885Reactome: R-HSA-193048Reactome: R-HSA-194002Reactome: R-HSA-202040Reactome: R-HSA-209952Reactome: R-HSA-211976Reactome: R-HSA-375276Reactome: R-HSA-418555Reactome: R-HSA-418594Reactome: R-HSA-5579031Reactome: R-HSA-6785807Reactome: R-HSA-9615017 |
| Cellular Localisation | SecretedMelanocyte-Stimulating Hormone Alpha And Beta-Endorphin Are Stored In Separate Granules In Hypothalamic Pomc NeuronsSuggesting That Secretion May Be Under The Control Of Different Regulatory Mechanisms |
| Alternative ELISA Names | Pro-Opiomelanocortin ELISA kitPomc ELISA kitCorticotropin-Lipotropin Cleaved Into - Npp - Melanotropin Gamma ELISA kitGamma-Msh - Potential Peptide - Corticotropin ELISA kitAdrenocorticotropic Hormone ELISA kitActh - Melanocyte-Stimulating Hormone Alpha ELISA kitAlpha-Msh ELISA kitMelanotropin Alpha - Corticotropin-Like Intermediary Peptide ELISA kitClip - Lipotropin Beta ELISA kitBeta-Lph - Lipotropin Gamma ELISA kitGamma-Lph - Melanocyte-Stimulating Hormone Beta ELISA kitBeta-Msh ELISA kitMelanotropin Beta - Beta-Endorphin - Met-Enkephalin ELISA kitPOMC ELISA kit |
| Specificity | This kit recognizes αMSH in samples. No significant cross-reactivity or interference between αMSH and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8℃ |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-106 | 99 |
| EDTA plasma(n=8) | 94-107 | 99 |
| Cell culture media(n=8) | 89-101 | 95 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.50 | 0.90 | 4.70 | 0.50 | 0.90 | 4.50 |
| Standard deviation | 0.04 | 0.04 | 0.22 | 0.03 | 0.04 | 0.15 |
| CV (%) | 8.00 | 4.44 | 4.68 | 6.00 | 4.44 | 3.33 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
