• Human Alpha MSH (Alpha-Melanocyte Stimulating Hormone) Competitive ELISA (STJE0003693)

Human Alpha MSH (Alpha-Melanocyte Stimulating Hormone) Competitive ELISA (STJE0003693)

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STJE0003693

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.09ng/mL
Detection Limit: 0.16~10ng/mL
Short Description: This Alpha MSH Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 2.5h
Detection: Colormetric
Gene Symbol: POMC
Gene ID: 5443
Uniprot ID: COLI_HUMAN
Specificity: This kit recognizes Human Alpha MSH in samples. No significant cross-reactivity or interference between Human Alpha MSH and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Specific enzymatic cleavages at paired basic residues yield the different active peptides. O-glycosylated.reducing sugar is probably N-acetylgalactosamine.
Function Corticotropin: Stimulates the adrenal glands to release cortisol. Melanocyte-stimulating hormone alpha: Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes. Melanocyte-stimulating hormone beta: Increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin: Endogenous orexigenic opiate. Met-enkephalin: Endogenous opiate.
Protein Name Pro-Opiomelanocortin
Pomc
Corticotropin-Lipotropin Cleaved Into - Npp - Melanotropin Gamma
Gamma-Msh - Potential Peptide - Corticotropin
Adrenocorticotropic Hormone
Acth - Melanocyte-Stimulating Hormone Alpha
Alpha-Msh
Melanotropin Alpha - Corticotropin-Like Intermediary Peptide
Clip - Lipotropin Beta
Beta-Lph - Lipotropin Gamma
Gamma-Lph - Melanocyte-Stimulating Hormone Beta
Beta-Msh
Melanotropin Beta - Beta-Endorphin - Met-Enkephalin
Database Links Reactome: R-HSA-111885
Reactome: R-HSA-193048
Reactome: R-HSA-194002
Reactome: R-HSA-202040
Reactome: R-HSA-209952
Reactome: R-HSA-211976
Reactome: R-HSA-375276
Reactome: R-HSA-418555
Reactome: R-HSA-418594
Reactome: R-HSA-5579031
Reactome: R-HSA-6785807
Reactome: R-HSA-9615017
Cellular Localisation Secreted
Melanocyte-Stimulating Hormone Alpha And Beta-Endorphin Are Stored In Separate Granules In Hypothalamic Pomc Neurons
Suggesting That Secretion May Be Under The Control Of Different Regulatory Mechanisms
Alternative ELISA Names Pro-Opiomelanocortin ELISA kit
Pomc ELISA kit
Corticotropin-Lipotropin Cleaved Into - Npp - Melanotropin Gamma ELISA kit
Gamma-Msh - Potential Peptide - Corticotropin ELISA kit
Adrenocorticotropic Hormone ELISA kit
Acth - Melanocyte-Stimulating Hormone Alpha ELISA kit
Alpha-Msh ELISA kit
Melanotropin Alpha - Corticotropin-Like Intermediary Peptide ELISA kit
Clip - Lipotropin Beta ELISA kit
Beta-Lph - Lipotropin Gamma ELISA kit
Gamma-Lph - Melanocyte-Stimulating Hormone Beta ELISA kit
Beta-Msh ELISA kit
Melanotropin Beta - Beta-Endorphin - Met-Enkephalin ELISA kit
POMC ELISA kit
Specificity This kit recognizes αMSH in samples. No significant cross-reactivity or interference between αMSH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-106 99
EDTA plasma(n=8) 94-107 99
Cell culture media(n=8) 89-101 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 0.50 0.90 4.70 0.50 0.90 4.50
Standard deviation 0.04 0.04 0.22 0.03 0.04 0.15
CV (%) 8.00 4.44 4.68 6.00 4.44 3.33
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance