| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.114ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This ALOXE3 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
ALOXE3 |
| Gene ID: |
59344 |
| Uniprot ID: |
LOXE3_HUMAN |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Predominantly expressed in skin. |
| Post Translational Modifications | |
| Function | Non-heme iron-containing lipoxygenase which is atypical in that it displays a prominent hydroperoxide isomerase activity and a reduced lipoxygenases activity. The hydroperoxide isomerase activity catalyzes the isomerization of hydroperoxides, derived from arachidonic and linoleic acid by ALOX12B, into hepoxilin-type epoxyalcohols and ketones. In presence of oxygen, oxygenates polyunsaturated fatty acids, including arachidonic acid, to produce fatty acid hydroperoxides. In the skin, acts downstream of ALOX12B on the linoleate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. In parallel, it may have a signaling function in barrier formation through the production of hepoxilins metabolites. Also plays a role in adipocyte differentiation through hepoxilin A3 and hepoxilin B3 production which in turn activate PPARG. Through the production of hepoxilins in the spinal cord, it may regulate inflammatory tactile allodynia. |
| Protein Name | Hydroperoxide Isomerase Aloxe3Epidermis-Type Lipoxygenase 3Epidermal Lox-3E-Lox-3Elox-3Hydroperoxy Dehydratase Aloxe3Hydroperoxy Icosatetraenoate DehydrataseHydroperoxy Icosatetraenoate Isomerase |
| Database Links | Reactome: R-HSA-2142712 |
| Cellular Localisation | Cytoplasm |
| Alternative ELISA Names | Hydroperoxide Isomerase Aloxe3 ELISA kitEpidermis-Type Lipoxygenase 3 ELISA kitEpidermal Lox-3 ELISA kitE-Lox-3 ELISA kitElox-3 ELISA kitHydroperoxy Dehydratase Aloxe3 ELISA kitHydroperoxy Icosatetraenoate Dehydratase ELISA kitHydroperoxy Icosatetraenoate Isomerase ELISA kitALOXE3 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
