| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.112ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This ALDOS Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
CYP11B2 |
| Gene ID: |
1585 |
| Uniprot ID: |
C11B2_HUMAN |
| Sample Type: |
serum, plasma, tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed sporadically in the zona glomerulosa (zG) of the adrenal cortex (conventional zonation), as well as in aldosterone-producing cell clusters (APCCs) composed of morphological zG cells in contact with the capsule (variegated zonation). |
| Post Translational Modifications | |
| Function | A cytochrome P450 monooxygenase that catalyzes the biosynthesis of aldosterone, the main mineralocorticoid in the human body responsible for salt and water homeostasis, thus involved in blood pressure regulation, arterial hypertension, and the development of heart failure. Catalyzes three sequential oxidative reactions of 11-deoxycorticosterone (21-hydroxyprogesterone), namely 11-beta hydroxylation, followed by two successive oxidations at C18 yielding 18-hydroxy and then 18-oxo intermediates (that would not leave the enzyme active site during the consecutive hydroxylation reactions), ending with the formation of aldosterone. Can also produce 18-hydroxycortisol and 18-oxocortisol, derived from successive oxidations of cortisol at C18, normally found at very low levels, but significantly increased in primary aldosteronism, the most common form of secondary hypertension. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Could also be involved in the androgen metabolic pathway (Probable). |
| Protein Name | Cytochrome P450 11b2 - MitochondrialAldosterone SynthaseAldosAldosterone-Synthesizing EnzymeCypxib2Corticosterone 18-Monooxygenase - Cyp11b2Cytochrome P-450aldoCytochrome P-450c18Steroid 11-Beta-Hydroxylase - Cyp11b2Steroid 18-Hydroxylase |
| Database Links | Reactome: R-HSA-193993Reactome: R-HSA-194002Reactome: R-HSA-211976Reactome: R-HSA-5579009 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane Protein |
| Alternative ELISA Names | Cytochrome P450 11b2 - Mitochondrial ELISA kitAldosterone Synthase ELISA kitAldos ELISA kitAldosterone-Synthesizing Enzyme ELISA kitCypxib2 ELISA kitCorticosterone 18-Monooxygenase - Cyp11b2 ELISA kitCytochrome P-450aldo ELISA kitCytochrome P-450c18 ELISA kitSteroid 11-Beta-Hydroxylase - Cyp11b2 ELISA kitSteroid 18-Hydroxylase ELISA kitCYP11B2 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
