| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human AHSP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
AHSP |
| Gene ID: |
51327 |
| Uniprot ID: |
AHSP_HUMAN |
| Specificity: |
This kit recognizes Human Alpha HSP in samples. No significant cross-reactivity or interference between Human Alpha HSP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in blood and bone marrow. |
| Function | Acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia. |
| Protein Name | Alpha-Hemoglobin-Stabilizing ProteinErythroid Differentiation-Related FactorErythroid-Associated Factor |
| Cellular Localisation | Cytoplasm |
| Alternative CLIA Names | Alpha-Hemoglobin-Stabilizing Protein CLIA kitErythroid Differentiation-Related Factor CLIA kitErythroid-Associated Factor CLIA kitAHSP CLIA kitEDRF CLIA kitERAF CLIA kit |
| Specificity | This kit recognizes Human αHSP in samples. No significant cross-reactivity or interference between Human αHSP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 96-113 | 104 |
| EDTA plasma(n=8) | 98-114 | 104 |
| Cell culture media(n=8) | 95-109 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 109.92 | 266.79 | 753.97 | 101.99 | 245.02 | 785.17 |
| Standard deviation | 11.56 | 29.35 | 56.77 | 8.50 | 27.74 | 68.23 |
| CV (%) | 10.52 | 11.00 | 7.53 | 8.33 | 11.32 | 8.69 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
