Human AGR2 (Anterior gradient protein 2 homolog) CLIA Kit (STJC0000187)

Human AGR2 (Anterior gradient protein 2 homolog) CLIA Kit (STJC0000187)

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STJC0000187
£180.50 - £462.50
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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description : This human AGR2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: AGR2
Gene ID: 10551
Uniprot ID: AGR2_HUMAN
Specificity: This kit recognizes Human AGR2 in samples. No significant cross-reactivity or interference between Human AGR2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
Function Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells. Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion.
Protein Name Anterior Gradient Protein 2 Homolog
Ag-2
Hag-2
Hpc8
Secreted Cement Gland Protein Xag-2 Homolog
Cellular Localisation Secreted
Endoplasmic Reticulum
Alternative CLIA Names Anterior Gradient Protein 2 Homolog CLIA kit
Ag-2 CLIA kit
Hag-2 CLIA kit
Hpc8 CLIA kit
Secreted Cement Gland Protein Xag-2 Homolog CLIA kit
AGR2 CLIA kit
AG2 CLIA kit
UNQ515 CLIA kit
PRO1030 CLIA kit
Specificity This kit recognizes Human AGR2 in samples. No significant cross-reactivity or interference between Human AGR2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-101 94
EDTA plasma(n=8) 84-98 91
Cell culture media(n=8) 92-109 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 110.65 170.57 861.73 114.70 186.33 864.08
Standard deviation 9.96 19.31 63.34 12.15 19.88 57.55
CV (%) 9.00 11.32 7.35 10.59 10.67 6.66

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