| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.47ng/mL |
| Detection Limit: |
0.78-50ng/mL |
| Short Description: |
This AFP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 6 months. Following first use any unused wells and reagents should be stored according to the components table in the manual. |
| Assay Time: |
1.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
AFP |
| Gene ID: |
174 |
| Uniprot ID: |
FETA_HUMAN |
| Specificity: |
This kit recognizes Human AFP in samples. No significant cross-reactivity or interference between Human AFP and analogues was observed. |
| Sample Type: |
Serum and plasma |
| Post Translational Modifications | Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated. |
| Function | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. |
| Protein Name | Alpha-FetoproteinAlpha-1-FetoproteinAlpha-Fetoglobulin |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Alpha-Fetoprotein ELISA kitAlpha-1-Fetoprotein ELISA kitAlpha-Fetoglobulin ELISA kitAFP ELISA kitHPAFP ELISA kit |
| Specificity | This kit recognizes αFP in samples. No significant cross-reactivity or interference between αFP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T | 2-8℃, 1 months |
| Reference Standard | 96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials | 2-8℃, use the reconstituted standard within 24h |
| Concentrated HRP Linked Detection Ab (100×) | 96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL | 2-8℃ (Protect from light) |
| Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL96T*5: 5 vials, 20 mL | 2-8℃ |
| HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL96T*5: 5 vials, 14 mL | |
| Concentrated Wash Buffer (25×) | 96T/48T/24T: 1 vial, 30 mL96T*5: 5 vials, 30 mL | |
| Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL | 2-8℃ |
| Plate Sealer | 96T/48T/24T: 5 pieces96T*5: 25 pieces | |
| Product Description | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-107 | 99 |
| EDTA plasma(n=8) | 87-98 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.40 | 6.58 | 17.73 | 2.29 | 6.46 | 16.31 |
| Standard deviation | 0.13 | 0.32 | 0.97 | 0.14 | 0.32 | 0.74 |
| CV (%) | 5.23 | 4.90 | 5.45 | 6.22 | 5.00 | 4.53 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
