| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This ADAM10 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
ADAM10 |
| Gene ID: |
102 |
| Uniprot ID: |
ADA10_HUMAN |
| Specificity: |
This kit recognizes Human ADAM10 in samples. No significant cross-reactivity or interference between Human ADAM10 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The precursor is cleaved by furin and PCSK7. |
| Function | Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3. Mediates the proteolytic cleavage of IL6R and IL11RA, leading to the release of secreted forms of IL6R and IL11RA. Enhances the cleavage of CHL1 by BACE1. Cleaves NRCAM. Cleaves TREM2, resulting in shedding of the TREM2 ectodomain. Involved in the development and maturation of glomerular and coronary vasculature. During development of the cochlear organ of Corti, promotes pillar cell separation by forming a ternary complex with CADH1 and EPHA4 and cleaving CADH1 at adherens junctions. May regulate the EFNA5-EPHA3 signaling. (Microbial infection) Promotes the cytotoxic activity of S.aureus hly by binding to the toxin at zonula adherens and promoting formation of toxin pores. |
| Protein Name | Disintegrin And Metalloproteinase Domain-Containing Protein 10Adam 10Cdw156Kuzbanian Protein HomologMammalian Disintegrin-MetalloproteaseCd Antigen Cd156c |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474228Reactome: R-HSA-177929Reactome: R-HSA-2122948Reactome: R-HSA-2644606Reactome: R-HSA-2660826Reactome: R-HSA-2691232Reactome: R-HSA-2894862Reactome: R-HSA-2979096Reactome: R-HSA-381426Reactome: R-HSA-3928665Reactome: R-HSA-6798695Reactome: R-HSA-8957275Reactome: R-HSA-9013507Reactome: R-HSA-9013700Reactome: R-HSA-977225 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinGolgi Apparatus MembraneCytoplasmic VesicleClathrin-Coated VesicleCell ProjectionAxonDendriteCell JunctionAdherens JunctionCytoplasmIs Localized In The Plasma Membrane But Is Also Expressed In The Golgi Apparatus And In Clathrin-Coated Vesicles Derived Likely From The GolgiDuring Long Term DepressionIt Is Recruited To The Cell Membrane By Dlg1The Immature Form Is Mainly Located Near Cytoplasmic Fibrillar StructuresWhile The Mature Form Is Predominantly Located At Zonula Adherens And The Cell MembraneThe Localization And Clustering Of Mature Adam10 To Zonula Adherens Is Regulated By AfdnTspan33Plekha7 And Pdzd11 |
| Alternative ELISA Names | Disintegrin And Metalloproteinase Domain-Containing Protein 10 ELISA kitAdam 10 ELISA kitCdw156 ELISA kitKuzbanian Protein Homolog ELISA kitMammalian Disintegrin-Metalloprotease ELISA kitCd Antigen Cd156c ELISA kitADAM10 ELISA kitKUZ ELISA kitMADM ELISA kit |
| Specificity | This kit recognizes Human ADAM10 in samples. No significant cross-reactivity or interference between Human ADAM10 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-95 | 90 |
| EDTA plasma (n=8) | 86-98 | 91 |
| Cell culture media (n=8) | 92-104 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 183.39 | 507.83 | 1750.20 | 190.85 | 537.66 | 1716.83 |
| Standard deviation | 10.73 | 28.34 | 52.86 | 11.09 | 22.96 | 93.91 |
| CV (%) | 5.85 | 5.58 | 3.02 | 5.81 | 4.27 | 5.47 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
