| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
7.7pg/mL |
| Detection Limit: |
15.6-1000pg/mL |
| Short Description: |
This ACE2 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
ACE2 |
| Gene ID: |
59272 |
| Uniprot ID: |
ACE2_HUMAN |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
serum, plasma or other biological fluids. |
| Tissue Specificity | Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells (at protein level). Expressed in enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in the renal proximal tubule and the small intestine (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system (at protein level). In lung, expressed at low levels in some alveolar type 2 cells, the expression seems to be individual-specific (at protein level). Expressed in nasal epithelial cells (at protein level). Coexpressed with TMPRSS2 within some lung alveolar type 2 cells, ileal absorptive enterocytes, intestinal epithelial cells, cornea, gallbladder and nasal goblet secretory cells. Coexpressed with TMPRSS4 within mature enterocytes. Isoform 2: Expressed in nasal and bronchial epithelial cells (at protein level). |
| Post Translational Modifications | N-glycosylation on Asn-90 may limit SARS infectivity. Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1. Phosphorylated. Phosphorylation at Tyr-781 probably inhibits interaction with AP2M1 and enables interactions with proteins containing SH2 domains. |
| Function | Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins (apelin-13, Pyr1apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency. In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity. (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. Isoform 2: Non-functional as a carboxypeptidase. Isoform 2: (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2. |
| Protein Name | Angiotensin-Converting Enzyme 2Angiotensin-Converting Enzyme HomologAcehAngiotensin-Converting Enzyme-Related CarboxypeptidaseAce-Related CarboxypeptidaseMetalloprotease Mprot15 Cleaved Into - Processed Angiotensin-Converting Enzyme 2 |
| Database Links | Reactome: R-HSA-2022377Reactome: R-HSA-9678110Reactome: R-HSA-9679191Reactome: R-HSA-9694614Reactome: R-HSA-9733458 |
| Cellular Localisation | Processed Angiotensin-Converting Enzyme 2: SecretedCell MembraneSingle-Pass Type I Membrane ProteinCytoplasmCell ProjectionCiliumApical Cell MembraneDetected In Both Cell Membrane And Cytoplasm In NeuronsIsoform 2: Apical Cell Membrane |
| Alternative ELISA Names | Angiotensin-Converting Enzyme 2 ELISA kitAngiotensin-Converting Enzyme Homolog ELISA kitAceh ELISA kitAngiotensin-Converting Enzyme-Related Carboxypeptidase ELISA kitAce-Related Carboxypeptidase ELISA kitMetalloprotease Mprot15 Cleaved Into - Processed Angiotensin-Converting Enzyme 2 ELISA kitACE2 ELISA kitUNQ868 ELISA kitPRO1885 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
