| Applications: |
ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
29pg/mL |
| Detection Limit: |
78.1-5000pg/mL |
| Short Description: |
This ABCA3 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
ABCA3 |
| Gene ID: |
21 |
| Uniprot ID: |
ABCA3_HUMAN |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed in brain, pancreas, skeletal muscle and heart. Highly expressed in the lung in an AT2-cell-specific manner. Weakly expressed in placenta, kidney and liver. Also expressed in medullary thyroid carcinoma cells (MTC) and in C-cell carcinoma. |
| Post Translational Modifications | N-glycosylated. Localization at intracellular vesicles is accompanied by processing of oligosaccharide from high mannose type to complex type. N-linked glycosylation at Asn-124 and Asn-140 is required for stability and efficient anterograde trafficking and prevents from proteasomal degradation. Proteolytically cleaved by CTSL and to a lower extent by CTSB within multivesicular bodies (MVB) and lamellar bodies (LB) leading to a mature form of 150 kDa. |
| Function | Catalyzes the ATP-dependent transport of phospholipids such as phosphatidylcholine and phosphoglycerol from the cytoplasm into the lumen side of lamellar bodies, in turn participates in the lamellar bodies biogenesis and homeostasis of pulmonary surfactant. Transports preferentially phosphatidylcholine containing short acyl chains. In addition plays a role as an efflux transporter of miltefosine across macrophage membranes and free cholesterol (FC) through intralumenal vesicles by removing FC from the cell as a component of surfactant and protects cells from free cholesterol toxicity. |
| Protein Name | Phospholipid-Transporting Atpase Abca3Abc-C TransporterAtp-Binding Cassette Sub-Family A Member 3Atp-Binding Cassette Transporter 3Atp-Binding Cassette 3Xenobiotic-Transporting Atpase Abca3 Cleaved Into - 150 Kda Mature Form |
| Database Links | Reactome: R-HSA-1369062Reactome: R-HSA-5683678Reactome: R-HSA-5683826Reactome: R-HSA-5688399 |
| Cellular Localisation | EndosomeMultivesicular Body MembraneMulti-Pass Membrane ProteinCytoplasmic Vesicle MembraneLate Endosome MembraneLysosome MembraneLocalized In The Limiting Membrane Of Lamellar Bodies In Lung Alveolar Type Ii CellsTrafficks Via The GolgiSorting Vesicles (Svs) And Late Endosome/Multivesicular Body Network Directly To The Outer Membrane Of Lamellar Bodies In At2 Lung Epithelial Cells Or To Lysosomes And Lysosomal-Related Organelles (Lros) In Other Cells Where Undergoes Proteolytic Cleavage And Oligosaccharide Processing From High Mannose Type To Complex TypeOligomers Formation Takes Place In A Post-Endoplasmic Reticulum Compartment |
| Alternative ELISA Names | Phospholipid-Transporting Atpase Abca3 ELISA kitAbc-C Transporter ELISA kitAtp-Binding Cassette Sub-Family A Member 3 ELISA kitAtp-Binding Cassette Transporter 3 ELISA kitAtp-Binding Cassette 3 ELISA kitXenobiotic-Transporting Atpase Abca3 Cleaved Into - 150 Kda Mature Form ELISA kitABCA3 ELISA kitABC3 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
