• Human a1AT (Alpha-1-Antitrypsin) Sandwich ELISA Kit (STJE0006787)

Human a1AT (Alpha-1-Antitrypsin) Sandwich ELISA Kit (STJE0006787)

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STJE0006787

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Applications: ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 11.37ng/mL
Detection Limit: 31.2-2000ng/mL
Short Description: This a1AT Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: SERPINA1
Gene ID: 5265
Uniprot ID: A1AT_HUMAN
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Tissue Specificity Ubiquitous. Expressed in leukocytes and plasma.
Post Translational Modifications N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)Man(alpha1-3)Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. (Microbial infection) Proteolytically processed by Staphylococcus aureus seryl, cysteinyl, and metallo-proteases.
Function Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT: Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
Protein Name Alpha-1-Antitrypsin
Alpha-1 Protease Inhibitor
Alpha-1-Antiproteinase
Serpin A1 Cleaved Into - Short Peptide From Aat
Spaat
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-204005
Reactome: R-HSA-381426
Reactome: R-HSA-5694530
Reactome: R-HSA-6798695
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Endoplasmic Reticulum
The S And Z Allele Are Not Secreted Effectively And Accumulate Intracellularly In The Endoplasmic Reticulum
Short Peptide From Aat: Secreted
Extracellular Space
Extracellular Matrix
Alternative ELISA Names Alpha-1-Antitrypsin ELISA kit
Alpha-1 Protease Inhibitor ELISA kit
Alpha-1-Antiproteinase ELISA kit
Serpin A1 Cleaved Into - Short Peptide From Aat ELISA kit
Spaat ELISA kit
SERPINA1 ELISA kit
AAT ELISA kit
PI ELISA kit
PRO0684 ELISA kit
PRO2209 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance