| Applications: | WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Glutamate Receptor 2 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | GRIA2 |
| Gene ID: | 2891 |
| Uniprot ID: | GRIA2_HUMAN |
| Post Translational Modifications | Palmitoylated. Depalmitoylated upon L-glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. Ubiquitinated by RNF167, leading to its degradation. Phosphorylation at Tyr-876 is required for interaction with IQSEC1 and ARF6 activation, which in turn triggers AMPAR internalization for persistent synaptic depression. N-glycosylated. |
| Function | Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate. Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting. |
| Peptide Name | Glutamate Receptor 2 Glur-2 Ampa-Selective Glutamate Receptor 2 Glur-B Glur-K2 Glutamate Receptor Ionotropic - Ampa 2 |
| Database Links | Reactome: R-HSA-399710 Reactome: R-HSA-416993 Reactome: R-HSA-438066 Reactome: R-HSA-9022699 Reactome: R-HSA-9620244 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Postsynaptic Cell Membrane Postsynaptic Density Membrane Interaction With Cacng2 Cnih2 And Cnih3 Promotes Cell Surface Expression Displays A Somatodendritic Localization And Is Excluded From Axons In Neurons |
| Alternative Peptide Names | Glutamate Receptor 2 protein Glur-2 protein Ampa-Selective Glutamate Receptor 2 protein Glur-B protein Glur-K2 protein Glutamate Receptor Ionotropic - Ampa 2 protein GRIA2 protein GluA2 protein GLUR2 protein |
Information sourced from Uniprot.org