• Western blot analysis of extracts of A-549 cells, using (STJ118466) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry of paraffin-embedded human liver using ZNRF3 antibody (STJ118466) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse spleen using ZNRF3 antibody (STJ118466) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat lung using ZNRF3 antibody (STJ118466) at dilution of 1:100 (40x lens).

Anti-ZNRF3 antibody (108-119) (STJ118466)

SKU:
STJ118466

£169.50 - £449.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZNRF3 (108-119) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZNRF3
Gene ID: 84133
Uniprot ID: ZNRF3_HUMAN
Immunogen Region: 108-119
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 108-119 of human ZNRF3 (NP_001193927.1).
Immunogen Sequence: IDQLNQGSEDPL
Function E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination and subsequent degradation of Wnt receptor complex components Frizzled and LRP6. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby restricting the size of the intestinal stem cell zone. Along with RSPO2 and RNF43, constitutes a master switch that governs limb specification.
Protein Name E3 Ubiquitin-Protein Ligase Znrf3
Ring Finger Protein 203
Ring-Type E3 Ubiquitin Transferase Znrf3
Zinc/Ring Finger Protein 3
Database Links Reactome: R-HSA-4641263
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Znrf3 antibody
Anti-Ring Finger Protein 203 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Znrf3 antibody
Anti-Zinc/Ring Finger Protein 3 antibody
Anti-ZNRF3 antibody
Anti-KIAA1133 antibody
Anti-RNF203 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance