• Immunofluorescence analysis of U-2 OS cells using ZNF581 Rabbit polyclonal antibody (STJ115128) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using ZNF581 Rabbit polyclonal antibody (STJ115128) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ZNF581 Rabbit polyclonal antibody (STJ115128) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ZNF581 in paraffin-embedded mouse kidney using ZNF581 Rabbit polyclonal antibody (STJ115128) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ZNF581 in paraffin-embedded human lung cancer using ZNF581 Rabbit polyclonal antibody (STJ115128) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ZNF581 in paraffin-embedded rat pancreas using ZNF581 Rabbit polyclonal antibody (STJ115128) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ZNF581 Rabbit polyclonal antibody (STJ115128) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ZNF581 antibody (1-90) (STJ115128)

SKU:
STJ115128

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZNF581 (1-90) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZNF581
Gene ID: 51545
Uniprot ID: ZN581_HUMAN
Immunogen Region: 1-90
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-90 of human ZNF581 (NP_057619.1).
Immunogen Sequence: MLVLPSPCPQPLAFSSVETM EGPPRRTCRSPEPGPSSSIG SPQASSPPRPNHYLLIDTQG VPYTVLVDEESQREPGASGA PGQKKCYSCP
Function May be involved in transcriptional regulation.
Protein Name Zinc Finger Protein 581
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Zinc Finger Protein 581 antibody
Anti-ZNF581 antibody
Anti-HSPC189 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance