• Western blot analysis of extracts of various cell lines, using ZNF277 antibody (STJ114691) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ZNF277 antibody (1-220) (STJ114691)

SKU:
STJ114691

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZNF277 (1-220) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZNF277
Gene ID: 11179
Uniprot ID: ZN277_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ZNF277 (NP_068834.2).
Immunogen Sequence: MAASKTQGAVARMQEDRDGS CSTVGGVGYGDSKDCILEPL SLPESPGGTTTLEGSPSVPC IFCEEHFPVAEQDKLLKHMI IEHKIVIADVKLVADFQRYI LYWRKRFTEQPITDFCSVIR INSTAPFEEQENYFLLCDVL PEDRILREELQKQRLREILE QQQQERNDTNFHGVCMFCNE EFLGNRSVILNHMAREHAFN IGLPDNIVNCNEFLCTLQKK
Function Probable transcription factor. Involved in modulation of cellular senescence.represses transcription of the tumor suppressor gene INK4A/ARF, perhaps acting via the Polycomb group (PcG) complex PRC1.
Protein Name Zinc Finger Protein 277
Nuclear Receptor-Interacting Factor 4
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Zinc Finger Protein 277 antibody
Anti-Nuclear Receptor-Interacting Factor 4 antibody
Anti-ZNF277 antibody
Anti-NRIF4 antibody
Anti-ZNF277P antibody

Information sourced from Uniprot.org

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