• Immunofluorescence analysis of U2OS cells using ZFYVE16 antibody (STJ110077) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of mouse kidney, using ZFYVE16 antibody (STJ110077) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ZFYVE16 antibody (1-280) (STJ110077)

SKU:
STJ110077

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ZFYVE16 (1-280) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZFYVE16
Gene ID: 9765
Uniprot ID: ZFY16_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ZFYVE16 (NP_055548.3).
Immunogen Sequence: MDSYFKAAVSDLDKLLDDFE QNPDEQDYLQDVQNAYDSNH CSVSSELASSQRTSLLPKDQ ECVNSCASSETSYGTNESSL NEKTLKGLTSIQNEKNVTGL DLLSSVDGGTSDEIQPLYMG RCSKPICDLISDMGNLVHAT NSEEDIKKLLPDDFKSNADS LIGLDLSSVSDTPCVSSTDH DSDTVREQQNDISSELQNRE IGGIKELGIKVDTTLSDSYN YSGTENLKDKKIFNQLESI
Tissue Specificity Widely expressed. Highly expressed in kidney, placenta and lung. Expressed at intermediate level in heart, brain, skeletal muscle, spleen and liver. Weakly expressed in colon, thymus and peripheral blood lymphocytes.
Function May be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes.
Protein Name Zinc Finger Fyve Domain-Containing Protein 16
Endofin
Endosome-Associated Fyve Domain Protein
Database Links Reactome: R-HSA-201451
Cellular Localisation Cytoplasm
Early Endosome Membrane
Peripheral Membrane Protein
Localized To Early Endosomes
Membrane-Associated
Probably Via Its Association With Phosphatidylinositol 3-Phosphate (Pi3p)
Alternative Antibody Names Anti-Zinc Finger Fyve Domain-Containing Protein 16 antibody
Anti-Endofin antibody
Anti-Endosome-Associated Fyve Domain Protein antibody
Anti-ZFYVE16 antibody
Anti-KIAA0305 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance