Western blot analysis of extracts of Raji cells, using ZC3H10 antibody (STJ115701) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ZC3H10 antibody (1-300) (STJ115701)

SKU:
STJ115701
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ZC3H10
Gene ID: 84872
Uniprot ID: ZC3HA_HUMAN
Immunogen Region: 1-300
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ZC3H10 (NP_116175.1).
Immunogen Sequence: MPDRDSYANGTGSSGGGPGG GGSEEASGAGVGSGGASSDA ICRDFLRNVCKRGKRCRYRH PDMSEVSNLGVSKNEFIFCH DFQNKECSRPNCRFIHGSKE DEDGYKKTGELPPRLRQKVA AGLGLSPADLPNGKEEVPIC RDFLKGDCQRGAKCKFRHLQ RDFEFDARGGGGTGGGSTGS VLPGRRHDLYDIYDLPDRGF EDHEPGPKRRRGGCCPPDGP HFESYEYSLAPPRGVECRL
Function Specific regulator of miRNA biogenesis. Binds, via the C3H1-type zinc finger domains, to the binding motif 5'-GCAGCGC-3' on microRNA pri-MIR143 and negatively regulates the processing to mature microRNA.
Protein Name Zinc Finger Ccch Domain-Containing Protein 10
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Zinc Finger Ccch Domain-Containing Protein 10 antibody
Anti-ZC3H10 antibody
Anti-ZC3HDC10 antibody

Information sourced from Uniprot.org

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