• Immunofluorescence analysis of PC-12 cells using YB-1/YB-1/YBX1 antibody (STJ118142) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using YB-1/YB-1/YBX1 antibody (STJ118142) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using YB-1/YB-1/YBX1 antibody (STJ118142) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using YB-1/YB-1/YBX1 antibody (STJ118142) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of HepG2 cells, using YB-1/YB-1/YBX1 antibody (STJ118142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-YBX1 antibody (100-200) (STJ118142)

SKU:
STJ118142

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-YBX1 (100-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: YBX1
Gene ID: 4904
Uniprot ID: YBOX1_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human YB-1/YB-1/YBX1 (NP_004550.2).
Immunogen Sequence: LRSVGDGETVEFDVVEGEKG AEAANVTGPGGVPVQGSKYA ADRNHYRRYPRRRGPPRNYQ QNYQNSESGEKNEGSESAPE GQAQQRRPYRRRRFPPYYMR R
Post Translational Modifications Ubiquitinated by RBBP6.leading to a decrease of YBX1 transactivational ability. Phosphorylated.increased by TGFB1 treatment (Ref.6). Phosphorylation by PKB/AKT1 reduces interaction with cytoplasmic mRNA. In the absence of phosphorylation the protein is retained in the cytoplasm. Cleaved by a 20S proteasomal protease in response to agents that damage DNA. Cleavage takes place in the absence of ubiquitination and ATP. The resulting N-terminal fragment accumulates in the nucleus.
Function DNA- and RNA-binding protein involved in various processes, such as translational repression, RNA stabilization, mRNA splicing, DNA repair and transcription regulation. Predominantly acts as a RNA-binding protein: binds preferentially to the 5'-CUCUGCG-3' RNA motif and specifically recognizes mRNA transcripts modified by C5-methylcytosine (m5C). Promotes mRNA stabilization: acts by binding to m5C-containing mRNAs and recruiting the mRNA stability maintainer ELAVL1, thereby preventing mRNA decay. Component of the CRD-mediated complex that promotes MYC mRNA stability. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. Plays a key role in RNA composition of extracellular exosomes by defining the sorting of small non-coding RNAs, such as tRNAs, Y RNAs, Vault RNAs and miRNAs. Probably sorts RNAs in exosomes by recognizing and binding C5-methylcytosine (m5C)-containing RNAs. Acts as a key effector of epidermal progenitors by preventing epidermal progenitor senescence: acts by regulating the translation of a senescence-associated subset of cytokine mRNAs, possibly by binding to m5C-containing mRNAs. Also involved in pre-mRNA alternative splicing regulation: binds to splice sites in pre-mRNA and regulates splice site selection. Binds to TSC22D1 transcripts, thereby inhibiting their translation and negatively regulating TGF-beta-mediated transcription of COL1A2. Also able to bind DNA: regulates transcription of the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA, suggesting a role in DNA repair. The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation.
Protein Name Y-Box-Binding Protein 1
Yb-1
Ccaat-Binding Transcription Factor I Subunit A
Cbf-A
Dna-Binding Protein B
Dbpb
Enhancer Factor I Subunit A
Efi-A
Nuclease-Sensitive Element-Binding Protein 1
Y-Box Transcription Factor
Database Links Reactome: R-HSA-2173796
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Reactome: R-HSA-72203
Reactome: R-HSA-877300
Reactome: R-HSA-9017802
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic Granule
Secreted
Extracellular Exosome
P-Body
Predominantly Cytoplasmic In Proliferating Cells
Cytotoxic Stress And Dna Damage Enhance Translocation To The Nucleus
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Shuttles Between Nucleus And Cytoplasm
Localized With Ddx1
Mbnl1 And Tial1 In Stress Granules Upon Stress
Secreted By Mesangial And Monocytic Cells After Inflammatory Challenges
Alternative Antibody Names Anti-Y-Box-Binding Protein 1 antibody
Anti-Yb-1 antibody
Anti-Ccaat-Binding Transcription Factor I Subunit A antibody
Anti-Cbf-A antibody
Anti-Dna-Binding Protein B antibody
Anti-Dbpb antibody
Anti-Enhancer Factor I Subunit A antibody
Anti-Efi-A antibody
Anti-Nuclease-Sensitive Element-Binding Protein 1 antibody
Anti-Y-Box Transcription Factor antibody
Anti-YBX1 antibody
Anti-NSEP1 antibody
Anti-YB1 antibody

Information sourced from Uniprot.org

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