• Immunofluorescence analysis of HeLa cells using TAZ Rabbit polyclonal antibody (STJ11104544) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using TAZ Rabbit polyclonal antibody (STJ11104544) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using TAZ Rabbit polyclonal antibody (STJ11104544) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-WWTR1 antibody (14-175) (STJ11104544)

SKU:
STJ11104544

Current Stock:
Host: Rabbit
Applications: IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Ww Domain-Containing Transcription Regulator Protein 1 (14-175) is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WWTR1
Gene ID: 25937
Uniprot ID: WWTR1_HUMAN
Immunogen Region: 14-175
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 14-175 of human TAZ (NP_056287.1).
Immunogen Sequence: GQQVIHVTQDLDTDLEALFN SVMNPKPSSWRKKILPESFF KEPDSGSHSRQSSTDSSGGH PGPRLAGGAQHVRSHSSPAS LQLGTGAGAAGSPAQQHAHL RQQSYDVTDELPLPPGWEMT FTATGQRYFLNHIEKITTWQ DPRKAMNQPLNHMNLHPAVS ST
Tissue Specificity Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.
Post Translational Modifications Phosphorylated by LATS2 and STK3/MST2. Phosphorylation by LATS2 results in creation of 14-3-3 binding sites, retention in the cytoplasm, and functional inactivation. Phosphorylation results in the inhibition of transcriptional coactivation through YWHAZ-mediated nuclear export. Ubiquitinated at Lys-46.leading to proteasomal degradation. Deubiquitinated and stabilized by UCHL1 at Lys-46.leading to osteoclastogenesis inhibition.
Function Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.
Protein Name Ww Domain-Containing Transcription Regulator Protein 1
Transcriptional Coactivator With Pdz-Binding Motif
Database Links Reactome: R-HSA-2028269
Reactome: R-HSA-2032785
Reactome: R-HSA-2173795
Reactome: R-HSA-2173796
Reactome: R-HSA-5578768
Reactome: R-HSA-8940973
Reactome: R-HSA-8951671
Reactome: R-HSA-9619665
Cellular Localisation Nucleus
Cytoplasm
Cell Membrane
Concentrates Along Specific Portions Of The Plasma Membrane
And Accumulates In Punctate Nuclear Bodies
When Phosphorylated
Is Retained In The Cytoplasm By Ywhaz
Can Be Retained In The Nucleus By Med15
Localized In The Cytoplasm In Areas Of Epithelial Cell High Density
At Blastocyst Stage Expressed In The Nucleus In Trophectodermal Cells
However Expressed In The Cytoplasm In The Inner Cell Mass
Alternative Antibody Names Anti-Ww Domain-Containing Transcription Regulator Protein 1 antibody
Anti-Transcriptional Coactivator With Pdz-Binding Motif antibody
Anti-WWTR1 antibody
Anti-TAZ antibody

Information sourced from Uniprot.org

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