• Western blot analysis of extracts of various cell lines, using WT1 rabbit polyclonal antibody (STJ11101198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
  • Immunofluorescence analysis of C6 cells using WT1 antibody (STJ11101198) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using WT1 antibody (STJ11101198) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using WT1 antibody (STJ11101198) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-WT1 antibody (1-302) (STJ11101198)

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STJ11101198

$228.83 - $606.83
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WT1 (1-302) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WT1
Gene ID: 7490
Uniprot ID: WT1_HUMAN
Immunogen Region: 1-302
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human WT1. (NP_001185480.1).
Immunogen Sequence: MEKGYSTVTFDGTPSYGHTP SHHAAQFPNHSFKHEDPMGQ QGSLGEQQYSVPPPVYGCHT PTDSCTGSQALLLRTPYSSD NLYQMTSQLECMTWNQMNLG ATLKGVAAGSSSSVKWTEGQ SNHSTGYESDNHTTPILCGA QYRIHTHGVFRGIQDVRRVP GVAPTLVRSASETSEKRPFM CAYPGCNKRYFKLSHLQMHS RKHTGEKPYQCDFKDCERRF SRSDQLKRHQRRHTGVKPF
Tissue Specificity Expressed in the kidney and a subset of hematopoietic cells.
Function Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
Protein Name Wilms Tumor Protein
Wt33
Database Links Reactome: R-HSA-9690406
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
Isoforms Lacking The Kts Motif Have A Diffuse Nuclear Location
Shuttles Between Nucleus And Cytoplasm
Isoform 1: Nucleus Speckle
Isoform 4: Nucleus
Nucleoplasm
Alternative Antibody Names Anti-Wilms Tumor Protein antibody
Anti-Wt33 antibody
Anti-WT1 antibody

Information sourced from Uniprot.org

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