• Western blot analysis of extracts of various cell lines, using WNT7B antibody (STJ110057) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of U2OS cells using WNT7B antibody (STJ110057) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-WNT7B antibody (120-349) (STJ110057)

SKU:
STJ110057

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WNT7B (120-349) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WNT7B
Gene ID: 7477
Uniprot ID: WNT7B_HUMAN
Immunogen Region: 120-349
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-349 of human WNT7B (NP_478679.1).
Immunogen Sequence: TAACSQGNLSNCGCDREKQG YYNQAEGWKWGGCSADVRYG IDFSRRFVDAREIKKNARRL MNLHNNEAGRKVLEDRMQLE CKCHGVSGSCTTKTCWTTLP KFREVGHLLKEKYNAAVQVE VVRASRLRQPTFLRIKQLRS YQKPMETDLVYIEKSPNYCE EDAATGSVGTQGRLCNRTSP GADGCDTMCCGRGYNTHQYT KVWQCNCKFHWCCFVKCNTC SERTEVFTCK
Tissue Specificity Moderately expressed in fetal brain, weakly expressed in fetal lung and kidney, and faintly expressed in adult brain, lung and prostate.
Post Translational Modifications Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Function Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal fusion of the chorion and the allantois during placenta development. Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation.
Protein Name Protein Wnt-7b
Database Links Reactome: R-HSA-3238698
Reactome: R-HSA-373080
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Protein Wnt-7b antibody
Anti-WNT7B antibody

Information sourced from Uniprot.org

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