• Immunofluorescence analysis of HeLa cells using IRF2 Rabbit polyclonal antibody (STJ24223). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of IRF2 in paraffin-embedded human gastric cancer using IRF2 Rabbit polyclonal antibody (STJ24223) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of IRF2 in paraffin-embedded rat brain using IRF2 Rabbit polyclonal antibody (STJ24223) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of IRF2 in paraffin-embedded rat heart using IRF2 Rabbit polyclonal antibody (STJ24223) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using (STJ24223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-IRF2 antibody (120-349) (STJ24223)

SKU:
STJ24223

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-IRF2 (120-349) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: IRF2
Gene ID: 3660
Uniprot ID: IRF2_HUMAN
Immunogen Region: 120-349
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-349 of human IRF2 (NP_002190.2).
Immunogen Sequence: KKGKKPKTEKEDKVKHIKQE PVESSLGLSNGVSDLSPEYA VLTSTIKNEVDSTVNIIVVG QSHLDSNIENQEIVTNPPDI CQVVEVTTESDEQPVSMSEL YPLQISPVSSYAESETTDSV PSDEESAEGRPHWRKRNIEG KQYLSNMGTRGSYLLPGMAS FVTSNKPDLQVTIKEESNPV PYNSSWPPFQDLPLSSSMTP ASSSSRPDRETRASVIKKTS DITQARVKSC
Tissue Specificity Expressed throughout the epithelium of the colon. Also expressed in lamina propria.
Post Translational Modifications Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity. The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation with SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter.
Function Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.
Protein Name Interferon Regulatory Factor 2
Irf-2
Database Links Reactome: R-HSA-5620971
Reactome: R-HSA-877300
Reactome: R-HSA-909733
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Interferon Regulatory Factor 2 antibody
Anti-Irf-2 antibody
Anti-IRF2 antibody

Information sourced from Uniprot.org

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