• Western blot analysis of extracts of various cell lines, using WNT3 antibody (STJ111660) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using WNT3 Rabbit polyclonal antibody (STJ111660) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-WNT3 antibody (166-355) (STJ111660)

SKU:
STJ111660

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WNT3 (166-355) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WNT3
Gene ID: 7473
Uniprot ID: WNT3_HUMAN
Immunogen Region: 166-355
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 166-355 of human WNT3 (NP_110380.1).
Immunogen Sequence: VLVSREFADARENRPDARSA MNKHNNEAGRTTILDHMHLK CKCHGLSGSCEVKTCWWAQP DFRAIGDFLKDKYDSASEMV VEKHRESRGWVETLRAKYSL FKPPTERDLVYYENSPNFCE PNPETGSFGTRDRTCNVTSH GIDGCDLLCCGRGHNTRTEK RKEKCHCIFHWCCYVSCQEC IRIYDVHTCK
Post Translational Modifications Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Function Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family. Required for normal gastrulation, formation of the primitive streak, and for the formation of the mesoderm during early embryogenesis. Required for normal formation of the apical ectodermal ridge. Required for normal embryonic development, and especially for limb development.
Protein Name Proto-Oncogene Wnt-3
Proto-Oncogene Int-4 Homolog
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3238698
Reactome: R-HSA-373080
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Proto-Oncogene Wnt-3 antibody
Anti-Proto-Oncogene Int-4 Homolog antibody
Anti-WNT3 antibody
Anti-INT4 antibody

Information sourced from Uniprot.org

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