• Immunofluorescence analysis of U-2 OS cells using WNT10B Rabbit polyclonal antibody (STJ119128) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using WNT10B Rabbit polyclonal antibody (STJ119128) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using WNT10B Rabbit polyclonal antibody (STJ119128) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of WNT10B in paraffin-embedded human colon carcinoma using WNT10B Rabbit polyclonal antibody (STJ119128) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of WNT10B in paraffin-embedded rat ovary using WNT10B Rabbit polyclonal antibody (STJ119128) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa cells, using WNT10B Rabbit polyclonal antibody (STJ119128) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-WNT10B antibody (260-340) (STJ119128)

SKU:
STJ119128

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-WNT10B (260-340) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WNT10B
Gene ID: 7480
Uniprot ID: WN10B_HUMAN
Immunogen Region: 260-340
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 260-340 of human WNT10B (NP_003385.2).
Immunogen Sequence: TCWRAAPEFRAVGAALRERL GRAIFIDTHNRNSGAFQPRL RPRRLSGELVYFEKSPDFCE RDPTMGSPGTRGRACNKTSR L
Tissue Specificity Detected in most adult tissues. Highest levels were found in heart and skeletal muscle. Low levels are found in brain.
Post Translational Modifications Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Function Member of the Wnt ligand gene family that encodes for secreted proteins, which activate the Wnt signaling cascade. Specifically activates canonical Wnt/beta-catenin signaling and thus triggers beta-catenin/LEF/TCF-mediated transcriptional programs. Involved in signaling networks controlling stemness, pluripotency and cell fate decisions. Acts in the immune system, mammary gland, adipose tissue, bone and skin.
Protein Name Protein Wnt-10b
Protein Wnt-12
Database Links Reactome: R-HSA-3238698
Reactome: R-HSA-373080
Reactome: R-HSA-381340
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Protein Wnt-10b antibody
Anti-Protein Wnt-12 antibody
Anti-WNT10B antibody
Anti-WNT12 antibody

Information sourced from Uniprot.org

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