• Immunofluorescence analysis of mouse spleen using WASP Rabbit monoclonal antibody (STJ11102268) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat spleen using WASP Rabbit monoclonal antibody (STJ11102268) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Jurkat cells using WASP Rabbit monoclonal antibody (STJ11102268) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Mouse liver, using WASP Rabbit monoclonal antibody (STJ11102268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using WASP Rabbit monoclonal antibody (STJ11102268) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-WAS antibody (1-100) [S8MR] (STJ11102268)

SKU:
STJ11102268

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-WASP (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WAS
Gene ID: 7454
Uniprot ID: WASP_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human WASP (P42768).
Immunogen Sequence: MSGGPMGGRPGGRGAPAVQQ NIPSTLLQDHENQRLFEMLG RKCLTLATAVVQLYLALPPG AEHWTKEHCGAVCFVKDNPQ KSYFIRLYGLQAGRLLWEQE
Tissue Specificity Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
Post Translational Modifications Phosphorylated at Tyr-291 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-291 enhances WAS activity in promoting actin polymerization and filopodia formation.
Function Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
Protein Name Actin Nucleation-Promoting Factor Was
Wiskott-Aldrich Syndrome Protein
Wasp
Database Links Reactome: R-HSA-202433
Reactome: R-HSA-2029482
Reactome: R-HSA-5663213
Reactome: R-HSA-9013148
Reactome: R-HSA-9013149
Reactome: R-HSA-9013409
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cytoskeleton
Nucleus
Alternative Antibody Names Anti-Actin Nucleation-Promoting Factor Was antibody
Anti-Wiskott-Aldrich Syndrome Protein antibody
Anti-Wasp antibody
Anti-WAS antibody
Anti-IMD2 antibody

Information sourced from Uniprot.org

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