| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-VPS4B (1-130) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
VPS4B |
| Gene ID: |
9525 |
| Uniprot ID: |
VPS4B_HUMAN |
| Immunogen Region: |
1-130 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human VPS4B (NP_004860.2). |
| Immunogen Sequence: |
MSSTSPNLQKAIDLASKAAQ EDKAGNYEEALQLYQHAVQY FLHVVKYEAQGDKAKQSIRA KCTEYLDRAEKLKEYLKNKE KKAQKPVKEGQPSPADEKGN DSDGEGESDDPEKKKLQNQL QGAIVIERPN |
| Tissue Specificity | Ubiquitously expressed. |
| Function | Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their ATP-dependent disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan. (Microbial infection) In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). |
| Protein Name | Vacuolar Protein Sorting-Associated Protein 4bCell Migration-Inducing Gene 1 ProteinSuppressor Of K(+ Transport Growth Defect 1Protein Skd1 |
| Database Links | Reactome: R-HSA-162588Reactome: R-HSA-917729 |
| Cellular Localisation | Late Endosome MembranePeripheral Membrane ProteinMembrane-Associated In The Prevacuolar Endosomal CompartmentLocalized In Hiv-1 Particles Purified From Acutely Infected Cells |
| Alternative Antibody Names | Anti-Vacuolar Protein Sorting-Associated Protein 4b antibodyAnti-Cell Migration-Inducing Gene 1 Protein antibodyAnti-Suppressor Of K(+ Transport Growth Defect 1 antibodyAnti-Protein Skd1 antibodyAnti-VPS4B antibodyAnti-SKD1 antibodyAnti-VPS42 antibodyAnti-MIG1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
