• Western blot analysis of extracts of various cell lines, using VCAM1 antibody (STJ11102824) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of RAW264. 7 cells, using VCAM1 antibody (STJ11102824) at 1:500 dilution. RAW264. 7 cells were treated by TNF-Alpha (20 ng/ml) at 37 °C for 16 hours after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-VCAM1 antibody (480-630) (STJ11102824)

SKU:
STJ11102824

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-VCAM1 (480-630) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VCAM1
Gene ID: 7412
Uniprot ID: VCAM1_HUMAN
Immunogen Region: 480-630
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 480-630 of human VCAM1 (NP_001069.1).
Immunogen Sequence: ALVCQAKLHIDDMEFEPKQR QSTQTLYVNVAPRDTTVLVS PSSILEEGSSVNMTCLSQGF PAPKILWSRQLPNGELQPLS ENATLTLISTKMEDSGVYLC EGINQAGRSRKEVELIIQVT PKDIKLTAFPSESVKEGDTV IISCTCGNVPE
Tissue Specificity Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
Post Translational Modifications Cleaved by the metalloproteinase ADAM17 to generate the soluble form. Sialoglycoprotein. Ubiquitinated by TRIM65 via 'Lys-48'-linked ubiquitination.leading to proteasomal degradation.
Function Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation.
Protein Name Vascular Cell Adhesion Protein 1
V-Cam 1
Vcam-1
Incam-100
Cd Antigen Cd106 Cleaved Into - Soluble Vascular Cell Adhesion Molecule-1
Database Links Reactome: R-HSA-198933
Reactome: R-HSA-216083
Reactome: R-HSA-6785807
Reactome: R-HSA-877300
Cellular Localisation Vascular Cell Adhesion Protein 1: Cell Membrane
Single-Pass Type I Membrane Protein
Soluble Vascular Cell Adhesion Molecule-1: Secreted
Alternative Antibody Names Anti-Vascular Cell Adhesion Protein 1 antibody
Anti-V-Cam 1 antibody
Anti-Vcam-1 antibody
Anti-Incam-100 antibody
Anti-Cd Antigen Cd106 Cleaved Into - Soluble Vascular Cell Adhesion Molecule-1 antibody
Anti-VCAM1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance