• Immunofluorescence analysis of U-2 OS cells using VAMP1 Rabbit monoclonal antibody (STJ11101892) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using VAMP1 Rabbit monoclonal antibody (STJ11101892) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using VAMP1 Rabbit monoclonal antibody (STJ11101892) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of VAMP1 in paraffin-embedded human brain tissue using VAMP1 Rabbit monoclonal antibody (STJ11101892) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of VAMP1 in paraffin-embedded mouse brain tissue using VAMP1 Rabbit monoclonal antibody (STJ11101892) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of VAMP1 in paraffin-embedded mouse colon tissue using VAMP1 Rabbit monoclonal antibody (STJ11101892) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of VAMP1 in paraffin-embedded rat brain tissue using VAMP1 Rabbit monoclonal antibody (STJ11101892) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using VAMP1 Rabbit monoclonal antibody (STJ11101892) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-VAMP1 antibody (1-118) [S2MR] (STJ11101892)

SKU:
STJ11101892

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-VAMP1 (1-118) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: VAMP1
Gene ID: 6843
Uniprot ID: VAMP1_HUMAN
Immunogen Region: 1-118
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human VAMP1 (P23763).
Immunogen Sequence: MSAPAQPPAEGTEGTAPGGG PPGPPPNMTSNRRLQQTQAQ VEEVVDIIRVNVDKVLERDQ KLSELDDRADALQAGASQFE SSAAKLKRKYWWKNCKMMIM LGAICAIIVVVIVIYFFT
Tissue Specificity Nervous system, skeletal muscle and adipose tissue.
Post Translational Modifications (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type B (BoNT/B, botB) which probably hydrolyzes the 78-Gln-|-Phe-79 bond and inhibits neurotransmitter release. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which probably hydrolyzes the 61-Arg-|-Leu-62 bond and inhibits neurotransmitter release. BoNT/D has low catalytic activity on this protein due to its sequence. Note that humans are not known to be infected by C.botulinum type D. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which probably hydrolyzes the 60-Gln-|-Lys-61 bond and inhibits neurotransmitter release. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type X (BoNT/X) which probably hydrolyzes the 68-Arg-|-Ala-69 bond and inhibits neurotransmitter release. It remains unknown whether BoNT/X is ever produced, or what organisms it targets.
Function Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Protein Name Vesicle-Associated Membrane Protein 1
Vamp-1
Synaptobrevin-1
Database Links Reactome: R-HSA-5250955
Reactome: R-HSA-5250981
Reactome: R-HSA-5250989
Cellular Localisation Isoform 1: Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Single-Pass Type Iv Membrane Protein
Synapse
Synaptosome
Isoform 2: Cytoplasmic Vesicle Membrane
Isoform 3: Mitochondrion Outer Membrane
Alternative Antibody Names Anti-Vesicle-Associated Membrane Protein 1 antibody
Anti-Vamp-1 antibody
Anti-Synaptobrevin-1 antibody
Anti-VAMP1 antibody
Anti-SYB1 antibody

Information sourced from Uniprot.org

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