• Western blot analysis of extracts of various cell lines, using USP28 antibody (STJ112436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-USP28 antibody (430-580) (STJ112436)

SKU:
STJ112436

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-USP28 (430-580) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP28
Gene ID: 57646
Uniprot ID: UBP28_HUMAN
Immunogen Region: 430-580
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 430-580 of human USP28 (NP_065937.1).
Immunogen Sequence: VKYGSGPARFPLPDMLKYVI EFASTKPASESCPPESDTHM TLPLSSVHCSVSDQTSKEST STESSSQDVESTFSSPEDSL PKSKPLTSSRSSMEMPSQPA PRTVTDEEINFVKTCLQRWR SEIEQDIQDLKTCIASTTQT IEQMYCDPLLR
Post Translational Modifications Degraded upon nickel ion level or hypoxia exposure. Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR. Phosphorylated by PRKD1.
Function Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 28
Deubiquitinating Enzyme 28
Ubiquitin Thioesterase 28
Ubiquitin-Specific-Processing Protease 28
Database Links Reactome: R-HSA-5689880
Cellular Localisation Nucleus
Nucleoplasm
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 28 antibody
Anti-Deubiquitinating Enzyme 28 antibody
Anti-Ubiquitin Thioesterase 28 antibody
Anti-Ubiquitin-Specific-Processing Protease 28 antibody
Anti-USP28 antibody
Anti-KIAA1515 antibody

Information sourced from Uniprot.org

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