• Immunofluorescence analysis of HeLa cells using USP15 Polyclonal Antibody (STJ28869) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using USP15 antibody (STJ28869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-USP15 antibody (852-952) (STJ28869)

SKU:
STJ28869

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-USP15 (852-952) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USP15
Gene ID: 9958
Uniprot ID: UBP15_HUMAN
Immunogen Region: 852-952
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 852-952 of human USP15 (NP_006304.1).
Immunogen Sequence: SNHYGGMGGGHYTAFAKNKD DGKWYYFDDSSVSTASEDQI VSKAAYVLFYQRQDTFSGTG FFPLDRETKGASAATGIPLE SDEDSNDNDNDIENENCMHT N
Tissue Specificity Expressed in skeletal muscle, kidney, heart, placenta, liver, thymus, lung, and ovary, with little or no expression in other tissues.
Post Translational Modifications Phosphorylated. Phosphorylation protects against ubiquitination and subsequent degradation by the proteasome. Ubiquitinated, leading to degradation by the proteasome.
Function Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling, NF-kappa-B and RNF41/NRDP1-PRKN pathways. Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear: according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes. According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal. Able to mediate deubiquitination of monoubiquitinated substrates, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B. Acts as an inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes cleavage of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains attached by parkin on target proteins such as MFN2, thereby reducing parkin's ability to drive mitophagy. Acts as an associated component of COP9 signalosome complex (CSN) and regulates different pathways via this association: regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP. Involved in endosome organization by mediating deubiquitination of SQSTM1: ubiquitinated SQSTM1 forms a molecular bridge that restrains cognate vesicles in the perinuclear region and its deubiquitination releases target vesicles for fast transport into the cell periphery. Acts as a negative regulator of antifungal immunity by mediating 'Lys-27'-linked deubiquitination of CARD9, thereby inactivating CARD9. (Microbial infection) Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase 15
Deubiquitinating Enzyme 15
Ubiquitin Thioesterase 15
Ubiquitin-Specific-Processing Protease 15
Unph-2
Unph4
Database Links Reactome: R-HSA-2173788
Reactome: R-HSA-5689603
Reactome: R-HSA-5689880
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase 15 antibody
Anti-Deubiquitinating Enzyme 15 antibody
Anti-Ubiquitin Thioesterase 15 antibody
Anti-Ubiquitin-Specific-Processing Protease 15 antibody
Anti-Unph-2 antibody
Anti-Unph4 antibody
Anti-USP15 antibody
Anti-KIAA0529 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance