• Western blot analysis of extracts of various cell lines, using USE1 antibody (STJ115117) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of NIH-3T3 cells using USE1 Polyclonal Antibody (STJ115117) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-USE1 antibody (1-259) (STJ115117)

SKU:
STJ115117

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-USE1 (1-259) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: USE1
Gene ID: 55850
Uniprot ID: USE1_HUMAN
Immunogen Region: 1-259
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-259 of human USE1 (NP_060937.1).
Immunogen Sequence: MAASRLELNLVRLLSRCEAM AAEKRDPDEWRLEKYVGALE DMLQALKVHASKPASEVINE YSWKVDFLKGMLQAEKLTSS SEKALANQFLAPGRVPTTAR ERVPATKTVHLQSRARYTSE MRSELLGTDSAEPEMDVRKR TGVAGSQPVSEKQSAAELDL VLQRHQNLQEKLAEEMLGLA RSLKTNTLAAQSVIKKDNQT LSHSLKMADQNLEKLKTESE RLEQHTQKSVNWLLWAMLI
Function SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.
Protein Name Vesicle Transport Protein Use1
Putative Mapk-Activating Protein Pm26
Use1-Like Protein
P31
Database Links Reactome: R-HSA-6811434
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Iv Membrane Protein
Alternative Antibody Names Anti-Vesicle Transport Protein Use1 antibody
Anti-Putative Mapk-Activating Protein Pm26 antibody
Anti-Use1-Like Protein antibody
Anti-P31 antibody
Anti-USE1 antibody
Anti-USE1L antibody
Anti-MDS032 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance