• Immunohistochemistry analysis of paraffin-embedded mouse brain using UNG antibody (STJ29843) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using UNG antibody (STJ29843) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using UNG antibody (STJ29843) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-UNG antibody (1-313) (STJ29843)

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STJ29843

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UNG (1-313) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UNG
Gene ID: 7374
Uniprot ID: UNG_HUMAN
Immunogen Region: 1-313
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human UNG (NP_550433.1).
Immunogen Sequence: MIGQKTLYSFFSPSPARKRH APSPEPAVQGTGVAGVPEES GDAAAIPAKKAPAGQEEPGT PPSSPLSAEQLDRIQRNKAA ALLRLAARNVPVGFGESWKK HLSGEFGKPYFIKLMGFVAE ERKHYTVYPPPHQVFTWTQM CDIKDVKVVILGQDPYHGPN QAHGLCFSVQRPVPPPPSLE NIYKELSTDIEDFVHPGHGD LSGWAKQGVLLLNAVLTVRA HQANSHKERGWEQFTDAVV
Tissue Specificity Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.
Post Translational Modifications Isoform 1 is processed by cleavage of a transit peptide.
Function Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
Protein Name Uracil-Dna Glycosylase
Udg
Database Links Reactome: R-HSA-110328 P13051-1
Reactome: R-HSA-110329 P13051-1
Reactome: R-HSA-110357 P13051-1
Cellular Localisation Isoform 1: Mitochondrion
Isoform 2: Nucleus
Alternative Antibody Names Anti-Uracil-Dna Glycosylase antibody
Anti-Udg antibody
Anti-UNG antibody
Anti-DGU antibody
Anti-UNG1 antibody
Anti-UNG15 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance