| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | Uhrf2 |
| Gene ID: | 109113 |
| Uniprot ID: | UHRF2_MOUSE |
| Immunogen Region: | 76-803 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 76-359 of mouse Uhrf2 (NP_659122.2). |
| Immunogen Sequence: | DSSLPSTSKQNDAQVKPSSH NPPKVKKTARGGSSSQPSTS ARTCLIDPGFGLYKVNELVD ARDVGLGAWFEAHIHSVTRA SDGHSRGKTPLKNGSSYKRT NGNVNHNSKENTNKLDNVPS TSNSDSVAADEDVIYHIEYD EYPESGILEMNVKDLRPRAR TILKWNELNVGDVVMVNYNV ENPGKRGFWYDAEITTLKTI SRTKKEVRVKVFLGGSEGTL NDCRVMSVDEIFKIEKPGA |
| Tissue Specificity | Mostly detected in several tissues, including the thymus, spleen, lung, adrenal gland, and ovary. In addition, found in several tissues in the brain (cerebellum, hippocampus, and cerebral cortex). |
| Post Translational Modifications | May be autoubiquitinated.which may lead to proteasomal degradation. Phosphorylated. Phosphorylation may be mediated by CDK2. Autosumoylated. |
| Function | E3 ubiquitin ligase that plays important roles in DNA methylation, histone modifications, cell cycle and DNA repair. Acts as a specific reader for 5-hydroxymethylcytosine (5hmC) and thereby recruits various substrates to these sites to ubiquitinate them. This activity also allows the maintenance of 5mC levels at specific genomic loci and regulates neuron-related gene expression. Participates in cell cycle regulation by ubiquitinating cyclins CCND1 and CCNE1 and thus inducing G1 arrest. Ubiquitinates also PCNP leading to its degradation by the proteasome. Plays an active role in DNA damage repair by ubiquitinating p21/CDKN1A leading to its proteasomal degradation. Promotes also DNA repair by acting as an interstrand cross-links (ICLs) sensor. Mechanistically, cooperates with UHRF1 to ensure recruitment of FANCD2 to ICLs, leading to FANCD2 monoubiquitination and subsequent activation. Contributes to UV-induced DNA damage response by physically interacting with ATR in response to irradiation, thereby promoting ATR activation. |
| Protein Name | E3 Ubiquitin-Protein Ligase Uhrf2 Nirf Np95-Like Ring Finger Protein Nuclear Protein 97 Nuclear Zinc Finger Protein Np97 Ring-Type E3 Ubiquitin Transferase Uhrf2 Ubiquitin-Like Phd And Ring Finger Domain-Containing Protein 2 Ubiquitin-Like-Containing Phd And Ring Finger Domains Protein 2 |
| Database Links | Reactome: R-MMU-3899300 |
| Cellular Localisation | Nucleus Chromosome Enriched At Genomic Loci That Are Enriched For 5-Hydroxy-Methylcytosine (5hmc) |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Uhrf2 antibody Anti-Nirf antibody Anti-Np95-Like Ring Finger Protein antibody Anti-Nuclear Protein 97 antibody Anti-Nuclear Zinc Finger Protein Np97 antibody Anti-Ring-Type E3 Ubiquitin Transferase Uhrf2 antibody Anti-Ubiquitin-Like Phd And Ring Finger Domain-Containing Protein 2 antibody Anti-Ubiquitin-Like-Containing Phd And Ring Finger Domains Protein 2 antibody Anti-Uhrf2 antibody Anti-Nirf antibody |
Information sourced from Uniprot.org