• Immunofluorescence analysis of NIH/3T3 cells using UCP3 Rabbit polyclonal antibody (STJ119263) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using UCP3 Rabbit polyclonal antibody (STJ119263) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using UCP3 Rabbit polyclonal antibody (STJ119263) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using UCP3 Rabbit polyclonal antibody (STJ119263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-UCP3 antibody (1-275) (STJ119263)

SKU:
STJ119263

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UCP3 (1-275) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UCP3
Gene ID: 7352
Uniprot ID: UCP3_HUMAN
Immunogen Region: 1-275
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human UCP3 (NP_073714.1).
Immunogen Sequence: MVGLKPSDVPPTMAVKFLGA GTAACFADLVTFPLDTAKVR LQIQGENQAVQTARLVQYRG VLGTILTMVRTEGPCSPYNG LVAGLQRQMSFASIRIGLYD SVKQVYTPKG
Tissue Specificity Only in skeletal muscle and heart. Also expressed in white and brown adipose tissues. Is more expressed in glycolytic than in oxidative skeletal muscles.
Function Putative transmembrane transporter that plays a role in mitochondrial metabolism via an as yet unclear mechanism. Originally, this mitochondrial protein was thought to act as a proton transmembrane transporter from the mitochondrial intermembrane space into the matrix, causing proton leaks through the inner mitochondrial membrane, thereby uncoupling mitochondrial membrane potential generation from ATP synthesis. However, this function is controversial and uncoupling may not be the function, or at least not the main function, but rather a consequence of more conventional metabolite transporter activity.
Protein Name Putative Mitochondrial Transporter Ucp3
Solute Carrier Family 25 Member 9
Uncoupling Protein-3
Ucp 3
Database Links Reactome: R-HSA-167826
Reactome: R-HSA-167827
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Putative Mitochondrial Transporter Ucp3 antibody
Anti-Solute Carrier Family 25 Member 9 antibody
Anti-Uncoupling Protein-3 antibody
Anti-Ucp 3 antibody
Anti-UCP3 antibody
Anti-SLC25A9 antibody

Information sourced from Uniprot.org

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