• Immunofluorescence analysis of NIH/3T3 cells using UBE2J1 Rabbit polyclonal antibody (STJ118286) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using UBE2J1 Rabbit polyclonal antibody (STJ118286) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using UBE2J1 Rabbit polyclonal antibody (STJ118286) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using UBE2J1 Rabbit polyclonal antibody (STJ118286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-UBE2J1 antibody (1-100) (STJ118286)

SKU:
STJ118286

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UBE2J1 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE2J1
Gene ID: 51465
Uniprot ID: UB2J1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBE2J1 (NP_057105.2).
Immunogen Sequence: METRYNLKSPAVKRLMKEAA ELKDPTDHYHAQPLEDNLFE WHFTVRGPPDSDFDGGVYHG RIVLPPEYPMKPPSIILLTA NGRFEVGKKICLSISGHHPE
Post Translational Modifications Phosphorylated at Ser-184 in a cytosolic stress-dependent manner by MAP kinase p38 MAPKAPK2. Phosphorylated UBE2J1 is rapidly ubiquitinated and subsequently degraded by the proteasome.
Function Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD) and is essential for cells to recover from ER stress. Plays a role in MAPKAPK2-dependent translational control of TNF-alpha synthesis. Acts also as a platform for perinuclear positioning of the endosomal system by mediating ubiquitination of SQSTM1 through interaction with the E3 ubiquitin-protein ligase RNF26. (Microbial infection) Promotes Dengue virus RNA replication by negatively regulating IFN-beta signaling and mediating 'Lys-48'-linked ubiquitination on IRF3.
Protein Name Ubiquitin-Conjugating Enzyme E2 J1
E2 Ubiquitin-Conjugating Enzyme J1
Non-Canonical Ubiquitin-Conjugating Enzyme 1
Ncube-1
Yeast Ubiquitin-Conjugating Enzyme Ubc6 Homolog E
Hsubc6e
Database Links Reactome: R-HSA-983168
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type Iv Membrane Protein
Alternative Antibody Names Anti-Ubiquitin-Conjugating Enzyme E2 J1 antibody
Anti-E2 Ubiquitin-Conjugating Enzyme J1 antibody
Anti-Non-Canonical Ubiquitin-Conjugating Enzyme 1 antibody
Anti-Ncube-1 antibody
Anti-Yeast Ubiquitin-Conjugating Enzyme Ubc6 Homolog E antibody
Anti-Hsubc6e antibody
Anti-UBE2J1 antibody
Anti-NCUBE1 antibody
Anti-CGI-76 antibody
Anti-HSPC153 antibody
Anti-HSPC205 antibody

Information sourced from Uniprot.org

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