• Immunohistochemistry analysis of UBE2B in paraffin-embedded rat brain tissue using UBE2B Rabbit monoclonal antibody (STJ11102688) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of UBE2B in paraffin-embedded mouse testis tissue using UBE2B Rabbit monoclonal antibody (STJ11102688) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of UBE2B in paraffin-embedded mouse spleen tissue using UBE2B Rabbit monoclonal antibody (STJ11102688) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of UBE2B in paraffin-embedded mouse colon tissue using UBE2B Rabbit monoclonal antibody (STJ11102688) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of UBE2B in paraffin-embedded human colon carcinoma tissue using UBE2B Rabbit monoclonal antibody (STJ11102688) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of UBE2B in paraffin-embedded human cervix cancer tissue using UBE2B Rabbit monoclonal antibody (STJ11102688) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of extracts of various cell lines, using UBE2B antibody (STJ11102688) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of extracts of Rat testis, using UBE2B antibody (STJ11102688) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-UBE2B antibody (53-152) [S8MR] (STJ11102688)

SKU:
STJ11102688

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-UBE2B (53-152) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBE2B
Gene ID: 7320
Uniprot ID: UBE2B_HUMAN
Immunogen Region: 53-152
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 53-152 of human UBE2B (P63146).
Immunogen Sequence: FKLVIEFSEEYPNKPPTVRF LSKMFHPNVYADGSICLDIL QNRWSPTYDVSSILTSIQSL LDEPNPNSPANSQAAQLYQE NKREYEKRVSAIVEQSWNDS
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
Protein Name Ubiquitin-Conjugating Enzyme E2 B
E2 Ubiquitin-Conjugating Enzyme B
Rad6 Homolog B
Hr6b
Hhr6b
Ubiquitin Carrier Protein B
Ubiquitin-Conjugating Enzyme E2-17 Kda
Ubiquitin-Protein Ligase B
Database Links Reactome: R-HSA-110314
Reactome: R-HSA-8866652
Reactome: R-HSA-8866654
Reactome: R-HSA-983168
Cellular Localisation Cell Membrane
Nucleus
In Peripheral Neurons
Expressed Both At The Plasma Membrane And In Nuclei
Alternative Antibody Names Anti-Ubiquitin-Conjugating Enzyme E2 B antibody
Anti-E2 Ubiquitin-Conjugating Enzyme B antibody
Anti-Rad6 Homolog B antibody
Anti-Hr6b antibody
Anti-Hhr6b antibody
Anti-Ubiquitin Carrier Protein B antibody
Anti-Ubiquitin-Conjugating Enzyme E2-17 Kda antibody
Anti-Ubiquitin-Protein Ligase B antibody
Anti-UBE2B antibody
Anti-RAD6B antibody

Information sourced from Uniprot.org

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