• Western blot analysis of lysates from wild type (WT) and TSC22D3 knockout (KO) 293T cells, using [KO Validated] TSC22D3 Rabbit polyclonal antibody (STJ11100787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-TSC22D3 antibody (58-134) (STJ11100787)

SKU:
STJ11100787

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TSC22D3 (58-134) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TSC22D3
Gene ID: 1831
Uniprot ID: T22D3_HUMAN
Immunogen Region: 58-134
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 58-134 of human TSC22D3 (NP_004080.2).
Immunogen Sequence: MDLVKNHLMYAVREEVEILK EQIRELVEKNSQLERENTLL KTLASPEQLEKFQSCLSPEE PAPESPQVPEAPGGSAV
Tissue Specificity Ubiquitously expressed, including in the fetal brain and liver. Expressed in brain, lung, spleen and skeletal muscle. Lower levels detected in heart and kidney. Not detected in the pancreas. In non-lymphoid tissues, in the absence of inflammation, the major source of constitutive expression is the macrophage lineage. Also expressed in cells from different hemopoietic cell lineages, including bone marrow cells, CD34+ stem cells, mature B- and T-cells, monocytes and granulocytes. Down-regulated in activated macrophages from inflammatory lesions of delayed-type hypersensitivity (DTH) reactions, such as in tuberculosis and in Crohn disease, whereas in Burkitt lymphoma, persists in macrophages involved in the phagocytosis of apoptotic malignant cells.
Function Protects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation and thereby NFKB1 DNA-binding activities. In vitro, suppresses AP-1 transcription factor complex DNA-binding activities. Isoform 1: Inhibits myogenic differentiation and mediates anti-myogenic effects of glucocorticoids by binding and regulating MYOD1 and HDAC1 transcriptional activity resulting in reduced expression of MYOG.
Protein Name Tsc22 Domain Family Protein 3
Dsip-Immunoreactive Peptide
Protein Dip
Hdip
Delta Sleep-Inducing Peptide Immunoreactor
Glucocorticoid-Induced Leucine Zipper Protein
Gilz
Tsc-22-Like Protein
Tsc-22-Related Protein
Tsc-22r
Database Links Reactome: R-HSA-2672351
Cellular Localisation Isoform 1: Cytoplasm
Nucleus
Localization Depends On Differentiation Status Of Myoblasts
In Undifferentiated Myoblasts
Localizes To The Cytoplasm
But In Differentiating Myoblast
Localizes To The Nucleus
Alternative Antibody Names Anti-Tsc22 Domain Family Protein 3 antibody
Anti-Dsip-Immunoreactive Peptide antibody
Anti-Protein Dip antibody
Anti-Hdip antibody
Anti-Delta Sleep-Inducing Peptide Immunoreactor antibody
Anti-Glucocorticoid-Induced Leucine Zipper Protein antibody
Anti-Gilz antibody
Anti-Tsc-22-Like Protein antibody
Anti-Tsc-22-Related Protein antibody
Anti-Tsc-22r antibody
Anti-TSC22D3 antibody
Anti-DSIPI antibody
Anti-GILZ antibody

Information sourced from Uniprot.org

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