• Western blot analysis of extracts of various cell lines, using TRIM9 antibody (STJ115834) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TRIM9 antibody (1-160) (STJ115834)

SKU:
STJ115834

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRIM9 (1-160) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRIM9
Gene ID: 114088
Uniprot ID: TRIM9_HUMAN
Immunogen Region: 1-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human TRIM9 (NP_055978.4).
Immunogen Sequence: MEEMEEELKCPVCGSFYREP IILPCSHNLCQACARNILVQ TPESESPQSHRAAGSGVSDY DYLDLDKMSLYSEADSGYGS YGGFASAPTTPCQKSPNGVR VFPPAMPPPATHLSPALAPV PRNSCITCPQCHRSLILDDR GLRGFPKNRVLEGVIDRYQQ
Tissue Specificity Brain. Highly expressed in the cerebral cortex (at protein level). Severely decreased in the affected brain areas in Parkinson disease and dementia with Lewy bodies.
Post Translational Modifications Auto-ubiquitinated. Poly-ubiquitinated in cultured cells, whereas it is monoubiquitinated in vitro.
Function E3 ubiquitin-protein ligase which ubiquitinates itself in cooperation with an E2 enzyme UBE2D2/UBC4 and serves as a targeting signal for proteasomal degradation. May play a role in regulation of neuronal functions and may also participate in the formation or breakdown of abnormal inclusions in neurodegenerative disorders. May act as a regulator of synaptic vesicle exocytosis by controlling the availability of SNAP25 for the SNARE complex formation.
Protein Name E3 Ubiquitin-Protein Ligase Trim9
Ring Finger Protein 91
Ring-Type E3 Ubiquitin Transferase Trim9
Tripartite Motif-Containing Protein 9
Database Links Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Cell Projection
Dendrite
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle
Synapse
Cytoskeleton
Enriched At Synaptic Terminals Where It Exists In A Soluble Form And A Synaptic Vesicle-Associated Form
Associated With The Cytoskeleton
Found In Proximal Dendrites Of Pyramidal Neurons In The Cerebral Cortex And Hippocampus
And Purkinje Cells In The Cerebellum
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Trim9 antibody
Anti-Ring Finger Protein 91 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Trim9 antibody
Anti-Tripartite Motif-Containing Protein 9 antibody
Anti-TRIM9 antibody
Anti-KIAA0282 antibody
Anti-RNF91 antibody

Information sourced from Uniprot.org

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