• Western blot analysis of various lysates using TRIM5 Rabbit polyclonal antibody (STJ11102889) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TRIM5 antibody (100-200) (STJ11102889)

SKU:
STJ11102889

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TRIM5 (100-200) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRIM5
Gene ID: 85363
Uniprot ID: TRIM5_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TRIM5 (NP_149083.2).
Immunogen Sequence: EKLLLFCQEDGKVICWLCER SQEHRGHHTFLTEEVAREYQ VKLQAALEMLRQKQQEAEEL EADIREEKASWKTQIQYDKT NVLADFEQLRDILDWEESNE L
Post Translational Modifications Degraded in a proteasome-independent fashion in the absence of viral infection but in a proteasome-dependent fashion following exposure to restriction sensitive virus. Autoubiquitinated in a RING finger- and UBE2D2-dependent manner. Monoubiquitinated by TRIM21. Deubiquitinated by Yersinia YopJ. Ubiquitination may not lead to proteasomal degradation.
Function Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV), equine infectious anemia virus (EIAV), simian immunodeficiency virus of macaques (SIVmac), feline immunodeficiency virus (FIV), and bovine immunodeficiency virus (BIV). Plays a role in regulating autophagy through activation of autophagy regulator BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2. Also plays a role in autophagy by acting as a selective autophagy receptor which recognizes and targets HIV-1 capsid protein p24 for autophagic destruction.
Protein Name Tripartite Motif-Containing Protein 5
Ring Finger Protein 88
Ring-Type E3 Ubiquitin Transferase Trim5
Database Links Reactome: R-HSA-877300
Cellular Localisation Cytoplasm
Nucleus
Predominantly Localizes In Cytoplasmic Bodies
Localization May Be Influenced By The Coexpression Of Other Trim Proteins
Hence Partial Nuclear Localization Is Observed In The Presence Of Trim22 Or Trim27
In Cytoplasmic Bodies
Colocalizes With Proteasomal Subunits And Sqstm1
Alternative Antibody Names Anti-Tripartite Motif-Containing Protein 5 antibody
Anti-Ring Finger Protein 88 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Trim5 antibody
Anti-TRIM5 antibody
Anti-RNF88 antibody

Information sourced from Uniprot.org

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