Host: | Rabbit |
Applications: | WB/ELISA |
Reactivity: | Human/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
Purification: | Affinity purification |
Concentration: | Lot specific |
Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | TRIM33 |
Gene ID: | 51592 |
Uniprot ID: | TRI33_HUMAN |
Immunogen Region: | 950-1090 |
Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 950-1090 of human TRIM33 (NP_056990.3). |
Immunogen Sequence: | KKGKTAQGLSPVDQRKCERL LLYLYCHELSIEFQEPVPAS IPNYYKIIKKPMDLSTVKKK LQKKHSQHYQIPDDFVADVR LIFKNCERFNEMMKVVQVYA DTQEINLKADSEVAQAGKAV ALYFEDKLTEIYSDRTFAPL P |
Tissue Specificity | Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors. |
Post Translational Modifications | Sumoylated with SUMO1. |
Function | Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade). |
Protein Name | E3 Ubiquitin-Protein Ligase Trim33 Ectodermin Homolog Ret-Fused Gene 7 Protein Protein Rfg7 Ring-Type E3 Ubiquitin Transferase Trim33 Transcription Intermediary Factor 1-Gamma Tif1-Gamma Tripartite Motif-Containing Protein 33 |
Database Links | Reactome: R-HSA-2173795 Reactome: R-HSA-9754189 |
Cellular Localisation | Nucleus In Discrete Nuclear Dots Resembling Nuclear Bodies Localizes To Sites Of Dna Damage |
Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Trim33 antibody Anti-Ectodermin Homolog antibody Anti-Ret-Fused Gene 7 Protein antibody Anti-Protein Rfg7 antibody Anti-Ring-Type E3 Ubiquitin Transferase Trim33 antibody Anti-Transcription Intermediary Factor 1-Gamma antibody Anti-Tif1-Gamma antibody Anti-Tripartite Motif-Containing Protein 33 antibody Anti-TRIM33 antibody Anti-KIAA1113 antibody Anti-RFG7 antibody Anti-TIF1G antibody |
Information sourced from Uniprot.org