• Western blot analysis of extracts from wild type (WT) and TRAF2 knockout (KO) 293T cells, using [KO Validated] TRAF2 Rabbit polyclonal antibody (STJ11105203) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of HeLa, using [KO Validated] TRAF2 Rabbit polyclonal antibody (STJ11105203) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TRAF2 antibody (STJ11105203)

SKU:
STJ11105203

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Tnf Receptor-Associated Factor 2 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TRAF2
Gene ID: 7186
Uniprot ID: TRAF2_HUMAN
Immunogen: Recombinant protein of human TRAF2.
Immunogen Sequence: DGCGKKKIPREKFQDHVKTC GKCRVPCRFHAIGCLETVEG EKQQEHEVQWLREHLAMLLS SVLEAKPLLGDQSHAGSELL QRCESLEKKTATFENIVCVL NREVERVAMTAEACSRQHRL DQDKIEALSSKVQQLERSIG LKDLAMADLEQKVLEMEAST Y
Post Translational Modifications Phosphorylated at several serine residues within the first 128 amino acid residues. Phosphorylated at Thr-117 in response to signaling via TNF and TNFRSF1A. Phosphorylation at Thr-117 is required for 'Lys-63'-linked polyubiquitination, but not for 'Lys-48'-linked polyubiquitination. Phosphorylation at Thr-117 is important for interaction with IKKA and IKKB, activation of IKK and subsequent activation of NF-kappa-B. Undergoes both 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination. Polyubiquitinated via 'Lys-63'-linked ubiquitin in response to TNF signaling.this requires prior phosphorylation at Thr-117. 'Lys-63'-linked polyubiquitination promotes TRAF2-mediated activation of NF-kappa-B. Can be polyubiquitinated at several Lys residues via 'Lys-48'-linked ubiquitin chains in response to TNF signaling, leading to proteasomal degradation. Autoubiquitinated, leading to its subsequent proteasomal degradation. Polyubiquitinated by BIRC2 and SIAH2, leading to its subsequent proteasomal degradation. Deubiquitinated by CYLD, a protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Ubiquination is inhibited by LRRC19.inhibits proteasomal degradation. Ubiquitinated at Lys-320 by the SCF(FBXL2) complex, leading to its degradation by the proteasome. Ubiquitinated by E3 ubiquitin-protein ligase complex containing FBXO7.leading to repression of NF-kappa-B signaling.
Function Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation.this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.
Protein Name Tnf Receptor-Associated Factor 2
E3 Ubiquitin-Protein Ligase Traf2
Ring-Type E3 Ubiquitin Transferase Traf2
Tumor Necrosis Factor Type 2 Receptor-Associated Protein 3
Database Links Reactome: R-HSA-140534
Reactome: R-HSA-3371378
Reactome: R-HSA-5213460
Reactome: R-HSA-5218900
Reactome: R-HSA-5357786
Reactome: R-HSA-5357905
Reactome: R-HSA-5357956
Reactome: R-HSA-5668541
Reactome: R-HSA-5675482
Reactome: R-HSA-5676594
Reactome: R-HSA-5689880
Reactome: R-HSA-69416
Reactome: R-HSA-75893
Reactome: R-HSA-933541
Reactome: R-HSA-933542
Reactome: R-HSA-9693928
Reactome: R-HSA-9758274
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Tnf Receptor-Associated Factor 2 antibody
Anti-E3 Ubiquitin-Protein Ligase Traf2 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Traf2 antibody
Anti-Tumor Necrosis Factor Type 2 Receptor-Associated Protein 3 antibody
Anti-TRAF2 antibody
Anti-TRAP3 antibody

Information sourced from Uniprot.org

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