| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S1MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:6000 IHC-P:1:200-1:800 IF/ICC:1:100-1:1000 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your sp |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | TOP1 |
| Gene ID: | 7150 |
| Uniprot ID: | TOP1_HUMAN |
| Immunogen Region: | 1-100 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNA topoisomerase I (TOP1) (P11387). |
| Immunogen Sequence: | MSGDHLHNDSQIEADFRLND SHKHKDKHKDREHRHKEHKK EKDREKSKHSNSEHKDSEKK HKEKEKTKHKDGSSEKHKDK HKDRDKEKRKEEKVRASGDA |
| Tissue Specificity | Endothelial cells. |
| Post Translational Modifications | Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment. Phosphorylation at Ser-506 by CK2 increases binding to supercoiled DNA and sensitivity to camptothecin. |
| Function | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the BMAL1 promoter. |
| Protein Name | Dna Topoisomerase 1 Dna Topoisomerase I |
| Database Links | Reactome: R-HSA-4615885 |
| Cellular Localisation | Nucleus Nucleolus Nucleoplasm Diffuse Nuclear Localization With Some Enrichment In Nucleoli On Cpt Treatment Cleared From Nucleoli Into Nucleoplasm Sumoylated Forms Found In Both Nucleoplasm And Nucleoli |
| Alternative Antibody Names | Anti-Dna Topoisomerase 1 antibody Anti-Dna Topoisomerase I antibody Anti-TOP1 antibody |
Information sourced from Uniprot.org